Molecular diagnostics
Apply advances in genetic analysis to fill crucial needs in disease detection, prenatal and reproductive care, and precision medicine.
The next level in diagnostic power
Benchtop IVD sequencing to enable labs to run diagnostic assays and develop a range of applications from targeted panels to WGS
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Work within a regulated sequencing workflow to conduct diagnostic tests while retaining the flexibility to develop your own laboratory developed test (LDT). With two operating modes, the NextSeq 550Dx Instrument enables labs to harness Illumina sequencing and array scanning methods to support a wide range of applications.
A simple three-step workflow, fast turnaround times (TAT), and integrated analysis software deliver accurate results for time-sensitive clinical IVD testing.
The NextSeq 550Dx Instrument and sequencing reagents are:
The NextSeq 550Dx Instrument is part of a robust IVD development toolkit when paired with the Illumina DNA Prep Enrichment Dx library preparation kit and the NextSeq 550Dx Reagent Kit. Develop your own assays with regulated components or benefit from a growing set of Illumina clinical assays.
Contact an Illumina representative for more information about IVD development partnerships.
Learn more about NGS for IVD assay development
Instrument specifications
The NextSeq 550Dx Instrument is easily configured, scalable, and user-friendly.
Benefit from a pipeline of IVD tests in development or design your own. Generate indexed libraries and create runs in Local Run Manager.
Add library to the ready-to-use NextSeq 550Dx reagent cartridge, load, and start run. Generate data < 35 hours.
Automatically launch data analysis with Local Run Manager and get results in a few hours. Leverage BaseSpace apps for additional analysis options.
Run IVD assays using targeted panels and a wide range of clinical research applications, including exome sequencing, targeted gene sequencing, whole-transcriptome sequencing, and cytogenomic arrays.
Apply advances in genetic analysis to fill crucial needs in disease detection, prenatal and reproductive care, and precision medicine.
Gain access to a robust IVD pipeline in partnership with Illumina and design custom NGS assays for your clinical lab.
NextSeq 550Dx Instrumenta |
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Run time | < 35 hr | 24 hr | ≤ 45 hr |
Maximum output per run | 120 Gb | ≥ 5 Gb | ≥ 3 Tb |
Maximum paired-end reads per run | > 300M | ≥ 15M | ≥ 20B |
Read length | 2 × 150 bp | 2 × 150 bpd | 2 × 150 bp |
Data quality | > 75% of bases higher than Q30 | ≥ 80% of bases higher than Q30 | ≥ 85% of bases higher than Q30 |
Data analysis offeringse | Dx Mode: Local Run Manager, dedicated DRAGEN server RUO Mode: Local Run Manager, Illumina Connected Analytics, on-premises DRAGEN server |
Dx Mode: Local Run Manager RUO Mode: Local Run Manager, Illumina Connected Analytics, on-premises DRAGEN server |
Dx Mode: Dedicated DRAGEN server RUO Mode: Dedicated DRAGEN server, BaseSpace Sequence Hub, Illumina Connected Analytics |
a. Libraries generated with TruSeq Custom Amplicon Kit Dx.
b. Libraries generated with Illumina DNA Prep with Enrichment Dx.
c. Based on a single S4 flow cell.
d. Maximum read length for the MiSeqDx Instrument is 2 x 300 bp, depending on the assay used.
e. A dedicated DRAGEN server is paired with an Illumina sequencing system and can be used to plan secondary analysis as part of sequencing run using the Illumina Run Manager. An on-premises DRAGEN server does not communicate directly with the sequencing system but can ingest and process data from any Illumina sequencing system via a command-line.
The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NextSeq 550Dx Instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx Instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic assays. The NextSeq 550Dx instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.
Contact us to learn more and ask questions to determine if the NextSeq 550Dx Instrument is right for your lab.
Your email address is never shared with third parties.