The AmpliSeq for Illumina Comprehensive Panel v3 offers coverage of key cancer genes, including kinase domains and genes involved in DNA repair.
The Comprehensive Panel v3 is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
The panel content spans hotspot regions, full-length genes*, copy number genes, and inter- and intragenic gene fusions. This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| MiniSeq System | 3 samples per run (high output; assumes minimum coverage of 500×) | 2 × 150 bp |
| MiSeq System | 3 samples per run with v3 reagents (assumes minimum coverage of 500×) | 2 × 150 bp |
| NextSeq 550 System | Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) | 2 × 150 bp |
| AmpliSeq for Illumina Comprehensive Panel v3 | TruSight Tumor 170 | TruSight Tumor 15 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Cancer Hotspot Panel v2 | |
|---|---|---|---|---|---|
| Assay Time | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | ~2 days (Library Prep) | 7 hours | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
| Cancer Type | Solid Tumor | Solid Tumor | Solid Tumor | Solid Tumor | Pan-Cancer, Solid Tumor |
| Content Specifications | DNA and RNA targets for 161 oncogenes | Probes enrich for full coding sequences of 170 genes. Calls single nucleotide variants, small insertions, and deletions in 151 genes, amplifications in 59 genes, and fusions plus splice variants in 55 genes. | Amplifies 250 amplicons from 15 genes associated with solid tumors | DNA and RNA targets for 52 oncogenes | Hotspot regions of 50 genes with known associations to cancer |
| Description | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. | Perform comprehensive somatic variant detection research in solid tumors using variant calling information from both DNA and RNA. | Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow. | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research into hotspot regions of 50 cancer-related genes. |
| Hands-On Time | < 1.5 hours | ~10.5 hours | 3.5 hours | < 1.5 hours | < 1.5 hours |
| Input Quantity | 1–100 ng (10 ng recommended per pool) | 40 ng DNA and/or RNA | 20 ng | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) |
| Method | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Target Enrichment, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing |
| Multiplexing | 96 dual index combinations | Up to 32-plex for DNA using both index sets, up to 16-plex for RNA | 1-24-plex | 96 dual index combinations | 96 dual index combinations |
| Nucleic Acid Type | DNA, RNA | RNA, DNA | DNA | DNA, RNA | DNA |
| Specialized Sample Types | FFPE Tissue | FFPE Tissue, Low-Input Samples | FFPE Tissue | FFPE Tissue | FFPE Tissue |
| Species Category | Human | Human | Human | Human | Human |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Ampliseq for Illumina Comprehensive Panel v3 Gene List
product_file | EXCEL < 1 MB
Data Sheet | HTML | 5 versions
NextSeq 550 amplicon sequencing solution
Application Note | PDF < 1 MB
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Comprehensive Panel v3 Checklist Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Comprehensive Panel v3 Documentation
AmpliSeq for Illumina Comprehensive Panel v3 Reference Guide Documentation
Index Adapters Pooling Guide Documentation
AmpliSeq for Illumina Comprehensive Panel v3 Consumables & Equipment Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
Comprehensive next-generation sequencing (NGS) assay that targets DNA and RNA variants from the same FFPE sample.
Targeted panel investigating the exonic regions of 409 genes with known associations to cancer.
Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.
Targeted research panel investigating somatic and germline variants in BRCA1 and BRCA2.
Targeted research panel investigating hotspot regions of 50 genes with known associations to cancer.
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
