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TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.
Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.
This TruSight Tumor panel offers:
3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.
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Instrument | Recommended Number of Samples | Read Length |
---|---|---|
MiniSeq System | Samples per run: mid output: 2-3, high output: 8 (based on 93.5% of the bases covered at 500x minimum) | 2 × 150 bp (max recommended) |
MiSeq System | 8 samples per run with v3 reagents (based on 93.5% of the bases covered at 500x minimum) | 2 × 151 bp (max recommended) |
AKT1 | GNA11 | NRAS |
BRAF | GNAQ | PDGFRA |
EGFR | KIT | PIK3CA |
ERBB2 | KRAS | RET |
FOXL2 | MET | TP53 |
Analysis of Cell-Free DNA with the TruSight Tumor 15 Assay
White Paper | PDF < 1 MB
Assessing the Quality of FFPE Samples for Preparation of TruSight® Tumor 15 Sequencing Libraries
White Paper | PDF < 1 MB
Analysis of TP53 Variants in Bone Marrow Aspirates With TruSight Tumor 15
White Paper | PDF < 1 MB
Data Sheet | PDF < 1 MB
Technical Note | PDF < 1 MB
TruSight Tumor 15 Data Analysis
Technical Note | PDF < 1 MB
Illumina Experiment Manager User Guide Documentation
TruSight Tumor 15 Checklist Documentation
TruSight Tumor 15 Reference Guide Documentation
TruSight Tumor 15 Support Documentation
TruSight Tumor 15 Consumables and Equipment Documentation
MiSeq Reporter TruSight Tumor 15 Workflow Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
Kit requires MiSeq Software v2.6 or higher and the Illumina Annotation Engine. Download files