Infinium Training
20015273
With the Infinium MethylationEPIC BeadChip Kit, researchers can interrogate over 850,000 methylation sites quantitatively across the genome at single-nucleotide resolution. Multiple samples, including FFPE, can be analyzed in parallel to deliver high-throughput power while minimizing the cost per sample.
Infinium HD array technology enables content selection independent of bias-associated limitations often associated with methylated DNA capture methods. The result is a pan-enhancer and coding region view of the methylome that can be used for epigenome-wide association studies on a variety of human tissues.
This kit includes content categories requested by methylation experts, such as:
MethylationEPIC data analysis is supported by the GenomeStudio Methylation Module. GenomeStudio Software enables researchers to effortlessly perform differential methylation analysis for small-scale studies. It features advanced visualization tools that enable researchers to view vast amounts of data in a single graph, such as heat maps, scatter plots, and line plots.
These tools and the GenomeStudio Genome Browser display valuable information such as chromosomal coordinates, percent GC, location in a CpG Island, and methylation β values.
For large-scale studies, there are many free analysis packages that work in the software framework R for normalization, and differential analysis of methylation data. Instructional videos explain the use of these data analysis packages.
Infinium MethylationEPIC Kit | TruSeq Methyl Capture EPIC Library Prep Kit | |
---|---|---|
Content Specifications | Targets 850,000 CpGs. Quantitatively interrogates selected methylation sites across the genome. | Targets over 3.3 million CpGs. Interrogates biologically important methylome targets. |
Description | Quantitative interrogation of selected methylation sites across the genome, offering high-throughput research capabilities at a minimal cost per sample. | Focused interrogation of a set of methylome targets for a balanced, cost-effective option that can support both screening studies and biomarker discovery research objectives. |
Input Quantity | 250 ng DNA | 500 ng human genomic DNA |
Method | Methylation Array | Methylation Sequencing , Targeted DNA Sequencing |
Number of Samples | 8 samples per array | Up to 12-plex |
Specialized Sample Types | FFPE Tissue, Low-Input Samples | Not FFPE-Compatible |
Species Category | Human | Human |
Technology | Microarray | Sequencing |
Technical Note | PDF1 MB
Analyzing FFPE Samples with Infinium Microarrays
Technical Note | PDF< 1 MB
Technical Note | PDF< 1 MB
STM Reagent Improves Robustness of Infinium Methylation Assay
Technical Note | PDF< 1 MB
Infinium Methylation BeadChip Application Note
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Infinium MethylationEPIC BeadChip Data Sheet | Illumina
Data Sheet | HTML
Infinium Assay Lab Setup and Procedures Guide Documentation
Infinium HD Assay for Methylation Lab Tracking Form Documentation
Infinium Assay Consumables and Equipment List Documentation
Infinium MethylationEPIC Kit Support Documentation
Infinium LiHa and Infinium RoMa Operating Manual Documentation
BeadArray Controls Reporter Software Guide Documentation
Infinium HD Methylation Assay Auto Workflow Checklist Documentation
Infinium HD Methylation Assay Manual Workflow Checklist Documentation
Infinium HD Methylation Assay Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
The Infinium MethylationEPIC Kit is the recommended replacement product for the HumanMethylation450 BeadChip Kit, which has been discontinued. Illumina remains committed to providing you with high-quality support and service.