With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Targeted approaches using next-generation sequencing (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA.
This comprehensive guide covers a broad range of NGS methods, from targeted resequencing to RNA-Seq, targeted metagenomics, epigenetics, and more.
Compared to broader approaches, such as whole-genome sequencing, targeted sequencing is a more cost-effective method for investigating areas of interest. Targeted resequencing enables researchers to focus on regions that are most likely to be involved in the phenotype under study, conserving resources and generating a smaller, more manageable data set. Targeted approaches can also deliver much higher coverage levels, allowing identification of variants that are rare and more expensive with whole-genome or Sanger sequencing.
Find out how targeted sequencing can help you discover more.
Explore common targeted NGS methods, from exome to amplicon sequencing and more.
Discover, validate, or screen genetic variants with targeted sequencing for various research areas, from cancer to microbiology and more.
Explore uses of targeted NGSWeb-based custom assay design tool makes it easy to optimize for target region coverage.
Custom targeted enrichment sequencing panels designed for content of interest.
A rapid, integrated library prep workflow for a wide range of applications, providing targeted resequencing with custom and fixed panels.
Illumina Cell-Free DNA Prep with Enrichment Fast, flexible library prep for highly sensitive mutation detection from cfDNA samples.
Cost-effective solution for diverse applications, integrates array scanning and benchtop sequencing.
Expand throughput with fast, robust performance, onboard informatics, and XLEAP-SBS chemistry.
Our most powerful sequencer for ultra-high-throughput projects, featuring up to 16 Tb output and 26 billion single reads per flow cell.
An accurate, efficient end-to-end secondary analysis solution for targeted panel NGS data.
Identifies somatic variants with sensitive detection of low-frequency alleles.
Highly accurate detection of a wide range of genetic variants.
NGS supports effective genomic surveillance strategies to identify and track infectious disease threats. Targeted sequencing can be used to monitor zoonotic or respiratory pathogens.
Use cutting-edge genotyping approaches to explore a broad range of genetic variants, such as single-nucleotide variants and copy number variants, and gain insight into disease etiology on a molecular level.
Target enrichment captures genomic regions of interest by using hybridization to target-specific biotinylated probes.