MiniSeq Sequencing System applications and methods

Accessible cross-method flexibility

Transition easily between sequencing methods for DNA and RNA applications

MiniSeq flow cell

Key applications and methods

Targeted DNA sequencing allows researchers to focus on a select set of genes or relevant gene regions using fixed panels of expert-selected content or custom, user-defined gene panels. By focusing the power of next-generation sequencing (NGS), targeted sequencing enables cost-effective and systematic variant detection with high accuracy and reproducibility.  

Read respiratory pathogen application note

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The MiniSeq System sequences up to 50 small genomes per run* to provide a detailed view of the genomes of the smallest organisms. Small whole-genome sequencing (WGS) enables comprehensive analysis of microbial or viral genomes for applications in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics. This approach does not require bacterial culture or labor-intensive cloning steps.

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*Based on 5 Mb genome, 30× coverage, 2 × 150 bp read length, Nextera XT Library Prep Kit, and MiniSeq Reagent High Output 300-cycle kit.

Targeted RNA sequencing (RNA-Seq) using the MiniSeq System enables gene expression measurement of dozens to thousands of targets simultaneously. The MiniSeq System supports user-defined panels to target genes, isoforms, splice junctions, cSNPs, and fusion genes. Predefined panels of expert-selected content target major signal transduction pathways and cellular processes. Sequence 1–384 targeted RNA samples per run.

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Based on 65 targets, 1000× coverage, 1 × 50 bp read length, TruSeq Targeted RNA Expression custom panel kit, and MiniSeq Reagent High Output 75-cycle kit.

More applications and methods

16S metagenomic sequencing

Sequence the 16S ribosomal RNA (rRNA) gene with a culture-free method to identify and compare bacteria from complex, difficult-to-study microbiomes or environments.

De novo sequencing

Enable fast, accurate characterization of novel genomes with no reference sequence available for any species.

Genotyping by sequencing (GBS)

Discover novel plant and animal SNPs and perform genotyping studies with a low-cost genetic screening method.

Germline mutation analysis

Assess genes with known or suspected associations with an increased hereditary risk of cancer.

Pathogen identification and analysis

Leverage NGS to detect and characterize viruses, bacteria, fungi, and parasites for research applications.

Small RNA discovery and profiling

Isolate and sequence small RNA species, such as microRNA, to study the role of noncoding RNA in gene silencing and posttranscriptional regulation.

Targeted gene sequencing

Select and sequence specific genes or genomic regions of interest using predesigned or custom designed panels for gene profiling studies.

How others use this instrument

See what’s possible with the MiniSeq System