Comprehensive coverage for epigenome-wide association studies

Quantitatively interrogate methylation sites across the genome at single-nucleotide resolution

Methylation Array Analysis

DNA methylation plays an important and dynamic role in regulating gene expression. With the MethylationEPIC BeadChip, researchers can interrogate over 850,000 methylation sites quantitatively across the genome at single-nucleotide resolution. Multiple samples, including FFPE, can be analyzed in parallel to deliver high-throughput power while minimizing the cost per sample.

Powered by the industry-leading Infinium assay, the MethylationEPIC BeadChip is ideal for epigenome wide association studies (EWAS). It offers comprehensive, expert-selected coverage, including 99% of RefSeq genes, 95% of CpG islands, high coverage of enhancer regions, and other content categories. With >90% of the original content from the Infinium HumanMethylation450K covered, the MethylationEPIC kit supports the next generation.

The MethylationEPIC BeadChip combines comprehensive coverage and high-throughput capabilities, making it ideal for EWAS. Additional advantages include:

  • Comprehensive genome-wide coverage (> 850,000 methylation sites)
    • CpG islands
    • Non-CpG and differentially methylated sites
    • FANTOM5 enhancers
    • ENCODE open chromatin
    • ENCODE transcription factor binding sites
    • miRNA promoter regions
  • Assay reproducibility
    • 98% for technical replicates
    • 98% for the same samples on the legacy HumanMethylation450K array vs. the MethylationEPIC array
    • >90% of the HumanMethylation450K content
  • User-friendly, streamlined workflow
  • Compatible with FFPE samples

The MethylationEPIC BeadChip follows a user-friendly, streamlined workflow, enabling processing of up to 96 samples simultaneously from low sample input (as little as 250 ng).

Offering quantitative measurement at the single-CpG-site level for normal and FFPE samples, this methylation microarray offers powerful resolution for understanding epigenetic changes.

Click on the below to view products for each workflow step.

MethylationEPIC BeadChip

PCR-Free, low sample input (as low as 250 ng), kit using Infinium I and Infinium II assay chemistry technologies.

iScan System

Supports high-throughput BeadChip processing, scanning hundreds to thousands of samples quickly and accurately.

GenomeStudio Software and Methylation Module

Effortless differential methylation analysis and display of chromosomal coordinates, % GC, location in CpG island, and methylation ß values.

Explore a variety of methods used to detect cytosine modifications, including methylation arrays, and learn more about the impact of cytosine methylation.

Read Guide
Field Guide to Methylation Methods
The Epigenetic Patterns of Exercise

Dr. Sundberg at the Karolinska Institute uses methylation arrays and RNA-Seq to reveal exercise-related epigenetic changes.

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Scouring The Epigenome For The Origins Of Dementia and Mental Illness

Dr. Mill at King’s College London uses methylation arrays to identify epigenetic changes that contribute to neurodegenerative disorders.

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Epigenetics Studies Uncover Obesity-Driven Methylation Signatures

Dr. Samani at University of Leicester uses methylation arrays to identify cell signaling disruption linked with high BMI.

Read Interview
Complex Disease Studies
Methylation Profiling for Complex Disease Studies

Methylation array studies enable researchers to understand the functional mechanisms at work in complex disease. Illumina offers complementary solutions for researchers studying these disorders. Learn more about complex disease research.

Epigenetics and Cancer
Cancer Epigenetics

Illumina array and sequencing solutions are ideal for detecting altered methylation patterns and epigenetic changes that provide insight into tumorigenic pathways and cancer progression. Learn more about cancer epigenetics.

Comprehensive molecular characterization of urothelial bladder carcinoma.

Nature 507 315-22 2014

Read Abstract

Epigenomic alterations define lethal CIMP-positive ependymomas of infancy.

Nature 506 445-50 2014

Read Abstract

Whole-genome sequence-based analysis of thyroid function.

Nat Commun 6 5681 2015

Read Abstract
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Methylation Sequencing
Methylation Sequencing

Methylation sequencing approaches offer higher density and flexibility in assessing epigenome changes.

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FastTrack Microarray Services
FastTrack Microarray Services

Illumina experts provide methylation array and genotyping services.

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Methylation Array Data Analysis
Methylation Array Data Analysis Tips from Experts

Experts describe their approaches to analyzing Illumina methylation array data.

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Epigenome-Wide Association Studies
Epigenome-Wide Association Studies

Epigenetic changes in newborns linked with maternal smoking during pregnancy.

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