The Infinium Methylation Assay provides quantitative array-based methylation measurement at the single-CpG-site level, offering high resolution for understanding epigenetic changes.
Genome-wide methylation analysis capabilities make this assay highly suitable for studying the biological role of DNA methylation in normal and diseased cells.
The Infinium Methylation Assay detects cytosine methylation at CpG islands based on highly multiplexed genotyping of bisulfite-converted genomic DNA. Upon treatment with bisulfite, unmethylated cytosine bases are converted to uracil, while methylated cytosine bases remain unchanged.
The assay interrogates these chemically differentiated loci using two site-specific probes, one designed for the methylated locus (M bead type), and another for the unmethylated locus (U bead type).
Single-base extension of the probes incorporates a labeled ddNTP, which is subsequently stained with a fluorescent reagent. The level of methylation for the interrogated locus can be determined by calculating the ratio of the fluorescent signals from the methylated vs. unmethylated sites.
The Infinium Methylation Assay is compatible with the iScan microarray imaging system.
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