The Respiratory Pathogen ID/AMR Enrichment Kit is a next-generation sequencing (NGS)-based respiratory pathogen panel that delivers highly sensitive, comprehensive pathogen detection and antimicrobial resistance (AMR) insights. The enrichment panel targets >280 respiratory pathogens, including SARS-CoV-2, Influenza and other viruses, bacteria, fungi, and more than 2000 AMR markers.
Powered by Explify RPIP Software, the flexible and scalable end-to-end workflow, coupled with broad identification of respiratory pathogens and AMR alleles in <24 hours, delivers a rapid, cost-effective solution for detecting respiratory tract infections in clinical research settings.
Features of this workflow:
Powerful, Simple Data Analysis with Explify RPIP
The Explify RPIP Analysis App offers a paired and tailored data analysis solution for the Illumina Respiratory Pathogen ID/AMR Enrichment Kit. The app provides information on sample composition, including host and microbial abundance, and proportion of targeted vs. untargeted sequences.
This app is available in BaseSpace Sequence Hub. Users can select up to 35 individual samples or project folders containing any number of samples for analysis. The app provides results in four different formats: (1) summary report (PDF), (2) SARS-CoV-2, Influenza A segment PA/NA/M1, known bacterial AMR markers, (3) SARS-CoV-2, Influenza A, Influenza B consensus genome sequence.
| Respiratory Pathogen ID/AMR Enrichment Panel Kit (Powered by Explify) | Respiratory Virus Oligo Panel | |
|---|---|---|
| Assay Time | < 9 hours library prep time | < 9 hours library prep time |
| Automation Capability | Liquid Handling Robots | Liquid Handling Robots |
| Description | A streamlined workflow using Respiratory Pathogen ID/AMR Panel (RPIP) with Illumina RNA Prep with Enrichment, sequencing on Illumina desktop systems, and powerful analysis using Explify RPIP Software allows researchers to identify respiratory infections and co-infections, identify antimicrobial resistance, and perform strain typing of critical pathogens (SARS-CoV-2 and Flu A/B viruses) to study viral evolution and transmission. | A streamlined workflow for detecting and analyzing SARS-CoV-2 and other common respiratory viruses using RNA Prep for Enrichment combined with Respiratory Virus Oligo Panel for library prep, proven Illumina sequencing, and simplified data analysis using the DRAGEN RNA Pathogen Detection App or Explify RVOP Software on BaseSpace Sequencing Hub. |
| Hands-on time | < 2 hours library prep time | < 2 hours library prep time |
| Multiplexing | Up to 384 samples in a single run with unique dual indexes | Up to 384 samples in a single run with unique dual indexes |
| Species Category | Bacteria, Fungal, Virus | Human, Virus |
| Species Details | Detects respiratory pathogens (180+ bacteria, 50+ fungi, and 40+ viruses, including SARS-CoV-2) and antimicrobial resistance alleles (1200+). | Detects common respiratory viruses, including recent flu strains and SARS-CoV-2. Includes human probes as a quality feature, human probes are included in every sample. |
| System Compatibility | MiniSeq, MiSeq, NextSeq 550 | MiniSeq, MiSeq, NextSeq 550 |
Illumina Respiratory Pathogen ID/AMR Panel Table
Product Information Sheet | PDF < 1 MB
Product Information Sheet | PDF | 2 versions
Application Note | PDF < 1 MB
Analytical performance of the Respiratory Pathogen ID/AMR Panel
Application Note | PDF < 1 MB
Illumina RNA Prep with Enrichment
Data Sheet | PDF < 1 MB
Illumina RNA Prep with Enrichment (L) Tagmentation Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
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Optimized chemistry to increase cluster density and read length, and improve sequencing quality scores, compared to earlier MiSeq reagent kit versions.
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Data management and simplified bioinformatics for labs getting started and for rapidly scaling next-generation sequencing (NGS) operations.
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
