Illumina DNA Prep with Exome 2.0 Plus Enrichment

A high-performance, fast, and complete whole-exome sequencing kit including library prep and hybridization reagents, a comprehensive exome probe panel, clean up/size selection beads, and indexes. Read More...
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Illumina® DNA Prep with Exome 2.0 Plus Enrichment, (S) Tagmentation Set B (96 samples, 12-plex)

20077595

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Illumina® DNA Prep with Exome 2.0 Plus Enrichment, (S) Tagmentation Set D (96 samples, 12-plex)

20077596

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Accessory Products
What accessories do I need?

Flex Lysis Reagent Kit (96 reactions)

20018706

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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

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Infinium MIDI Heatblock Insert

BD-60-601

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Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

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Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

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Illumina Purification Beads (IPB)

20060057

Price
 
 

Illumina Purification Beads (IPB)

20060058

Price
 
 

Product Highlights

Illumina DNA Prep with Exome 2.0 Plus Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES).

Focused, Comprehensive Coverage

Twist Bioscience for Illumina Exome 2.0 Plus Panel provides comprehensive coverage of exons and variants across public databases, maximizing the utility of your sequencing results.

Outstanding Enrichment Performance

Illumina DNA Prep with Exome 2.0 Plus Enrichment provides high on-target rates and uniform coverage enabling high multiplexing, in turn decreasing costs and increasing sample throughput.

Fast, Streamlined Workflow

Illumina DNA Prep with Exome 2.0 Plus Enrichment uses a fast, user-friendly workflow with built-in library yield normalization and a simplified, single hybridization protocol. Go from DNA to library prep, enrichment, and post-enrichment amplification in just 6.5 hours.

Single-Vendor End-to-End Solution

You and your lab can benefit from a WES solution with support from a single, reliable partner—starting from direct blood or saliva input, through library prep, enrichment, sequencing, analysis, interpretation, and reporting.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 2000 System Samples per run (by flow cell type): P2: 19, P3: 57 (based on 50× mean target coverage, 42M reads, 4.2 Gb of data) 2 × 101 bp
NovaSeq 6000 System Samples per run by flow cell type: SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes. 2 × 101 bp

Product Comparison

Illumina DNA Prep with Exome 2.0 Plus Enrichment Illumina DNA Prep with Enrichment TruSeq DNA Exome
Assay Time 6.5 hours ~6.5 hours 2.5 days
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Content Specifications 37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Custom: 0.5 - 15 Mb genomic content of interest.
Fixed panels: Content varies by panel.
Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Description A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing. A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.
Hands-On Time ~2 hours ~2 hours 6 hours
Input Quantity 50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide) 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). 100 ng genomic DNA
Mechanism of Action Bead-linked transposome and hybrid-capture chemistry Bead-bound transposomes and hybrid-capture chemistry Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes
Method Exome Sequencing, Target Enrichment, Targeted DNA Sequencing Custom Sequencing, Exome Sequencing, Target Enrichment, Targeted DNA Sequencing Exome Sequencing
Multiplexing Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible. Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. Up to 12-plex enrichment
Nucleic Acid Type DNA DNA DNA
On-Target Reads ~ 90% (ave.) based on average performance from internal testing ≥85% (for exome panel)* >80%
Specialized Sample Types Blood, Saliva Blood, FFPE Tissue, Saliva FFPE Tissue
Species Category Human Human, Other Human
System Compatibility HiSeq 2500, HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000 HiSeq 2500, HiSeq 3000, HiSeq 4000, iSeq 100, MiniSeq, MiSeq, MiSeqDx in Research Mode, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000 HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000
Technology Sequencing Sequencing Sequencing
Uniformity ≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing ≥90% (% coverage at 20x for 4 Gb; for exome panel) >85% (% coverage at 20x for 4 Gb)
Variant Class Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants

Supporting Data and Figures

 

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

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