Targeted panel that measures expression levels of >20,000 human RefSeq genes.
Assay time
Hands-on time
Input quantity
The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel is a highly multiplexed targeted resequencing assay for measuring expression levels of over 20,000 human RefSeq genes in a single assay.
The Transcriptome Human Gene Expression Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
| Assay time | 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) |
|---|---|
| Content specifications | > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome |
| Description | Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. |
| Hands-on time | < 1.5 hr |
| Input quantity | 1-100 ng RNA (10 ng recommended) |
| Instruments | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System |
| Mechanism of action | Multiplex PCR |
| Method | Exome sequencing, mRNA sequencing, Targeted RNA sequencing |
| Multiplexing | 96 dual index combinations |
| Nucleic acid type | RNA |
| Number of amplicons | 1 pool |
| Specialized sample types | Blood, FFPE tissue |
| Species category | Human |
| Technology | Sequencing |
| Variant class | Transcript variants |
The AmpliSeq for Illumina Transcriptome Human Gene Expression Panel workflow requires the Transcriptome Panel, Library PLUS kit, and index adapter. RNA workflow also requires cDNA Synthesis.
AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
With targeted resequencing, a subset of genes or a genomic region is isolated and sequenced, which can conserve lab resources.
Detect cancer gene expression and transcriptome changes and identify novel cancer transcripts with RNA next-generation sequencing (RNA-Seq).
mRNA sequencing (mRNA-Seq) using NGS can identify both known and novel transcripts, and measure transcript abundance.
| AmpliSeq for Illumina Transcriptome Human Gene Expression Panel | Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome | TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|---|---|
| Assay time | 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | ~7 hr | 11.5 hr | 11.5 hr |
| Content specifications | > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA |
| Description | Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons. | For the preparation of whole-transcriptome sequencing-ready libraries from low RNA inputs and low-quality samples that supports a wide range of RNA-Seq applications. Includes Ribo-Zero Plus for single-tube depletion of rRNA and globin RNA from multiple species to facilitate rich transcriptome analyses. | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. |
| Hands-on time | < 1.5 hr | < 3 hr | 5.5 hr | 5.5 hr |
| Input quantity | 1-100 ng RNA (10 ng recommended) | 1–1000 ng total RNA for standard-quality RNA samples. 10 ng total RNA minimum recommended for optimal performance and FFPE samples | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1 – 1 ug high-quality purified total RNA from blood |
| Instruments | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System |
| Mechanism of action | Multiplex PCR | Enzymatic rRNA depletion, ligation-based addition of adapters and indexes | Bead-based rRNA depletion, cDNA synthesis, and PCR | Bead-based rRNA depletion, cDNA synthesis, and PCR |
| Method | Exome sequencing, mRNA sequencing, Targeted RNA sequencing | Whole-transcriptome sequencing, Metatranscriptome sequencing | Whole-transcriptome sequencing | Whole-transcriptome sequencing |
| Nucleic acid type | RNA | RNA | RNA | RNA |
| Number of amplicons | 1 pool | |||
| Specialized sample types | Blood, FFPE tissue | Microbiome samples, Low-input samples, FFPE tissue | FFPE tissue | Blood |
| Species category | Human | Mouse, Human, Rat, Bacteria | Mouse, Human, Rat | Mouse, Human, Rat |
| Technology | Sequencing | Sequencing | Sequencing | Sequencing |
| Variant class | Transcript variants | Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants | Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants | Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants |
AmpliSeq™ Transcriptome Human Gene Expression Panel for Illumina®
20019170
Sufficient for 24 reactions when paired with AmpliSeq Library PLUS for Illumina. Purchase library prep, index adapters, and accessory products separately.
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AmpliSeq™ Library PLUS (24 Reactions) for Illumina®
20019101
Includes reagents for preparing 24 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®
20019102
Includes reagents for preparing 96 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®
20019103
Includes reagents for preparing 384 libraries. Purchase AmpliSeq for Illumina panel and index adapters separately.
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AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)
20019104
Includes 24 indexes sufficient for labeling 24 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A for Illumina®
20019105
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set B for Illumina®
20019106
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set C for Illumina®
20019107
Includes 96 indexes sufficient for labeling 96 samples. Purchase library prep and enrichment reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set D for Illumina®
20019167
Includes 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep reagents and probe panels separately.
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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)
20031676
Includes four sets of AmpliSeq CD Indexes for Illumina, Set A, Set B, Set C and Set D. Each set contains 96, 8 bp indexes sufficient for labeling 96 samples. Purchase library prep and probe panels separately.
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Ampliseq™ cDNA Synthesis for Illumina®
20022654
Includes reaction mix and enzyme blend to convert total RNA to cDNA when working with AmpliSeq for Illumina RNA Panels (eg, AmpliSeq for Illumina Immune Response, Immune Repertoire Plus, TCR beta Panel, Myeloid, Transcriptome Human Gene Expression, Focus, and Comprehensive Panel v3 as well as Custom or Community RNA Panels). Number of reactions vary by panel (eg, 100 reactions per kit for AmpliSeq for Illumina Immune Response, Focus, Comprehensive Panel v3, and Custom panels and 200 reactions per kit for AmpliSeq for Illumina Transcriptome Human Gene Expression, Myeloid, Immune Repertoire Plus, and TCR beta Panels).
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AmpliSeq™ for Illumina® Sample ID Panel
20019162
Includes eight single nucleotide polymorphism (SNP)-targeting primer pairs and one gender discriminating primer pair sufficient for 96 reactions when paired with AmpliSeq Library PLUS for Illumina. Enables quick and accurate sample identification. Purchase library prep, probe panels, and index adapters separately.
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AmpliSeq™ for Illumina® Direct FFPE DNA
20023378
Includes 24 reactions to prepare DNA from unstained, slide-mounted FFPE tissues for library construction using AmpliSeq for Illumina protocol, without the need for deparaffinization or DNA purification.
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AmpliSeq™ Library Equalizer for Illumina®
20019171
Includes beads and reagents for library normalization when preparing AmpliSeq for Illumina libraries for sequencing.
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