Create amplicon panels with the genes you want. AmpliSeq for Illumina On Demand panels provide a choice of > 5000 pretested genes with known content relevant for inherited disease research, including hereditary cancer, primary immunodeficiency, hearing loss, and muscular dystrophy.
AmpliSeq chemistry and Illumina next-generation sequencing (NGS) technology provide unmatched data quality, even from low-quality starting materials such as formalin-fixed, paraffin-embedded (FFPE) tissues.
| AmpliSeq for Illumina On-Demand | AmpliSeq for Illumina Custom DNA Panel | Illumina DNA Prep with Enrichment | |
|---|---|---|---|
| Content Specifications | Custom content from 1 (24 amplicons) to 500 (15,000 amplicons) genes | Custom content of interest - up to 5 Mb | Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
| Description | A custom research assay from >5000 pretested genes that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | A custom research assay that can be used to focus studies on specific genes, regions, or variants of interest with high accuracy. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. |
| Input Quantity | 1-100 ng DNA | 1–100 ng (10 ng recommended per pool) | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). |
| Mechanism of Action | Multiplex PCR | Multiplex PCR | Bead-bound transposomes and hybrid-capture chemistry |
| Method | Amplicon Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Custom Sequencing, Genotyping by Sequencing, Targeted DNA Sequencing | Custom Sequencing, Exome Sequencing, Target Enrichment, Targeted DNA Sequencing |
| Multiplexing | Up to 96-plex | Up to 96-plex | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. |
| Number of Reactions | 24 or 96 reactions | 750 or 3000 reactions, 750 or 3000 reactions | Custom workflow: 16 or 96 samples |
| Specialized Sample Types | Blood, FFPE Tissue | Blood, FFPE Tissue | Blood, FFPE Tissue, Saliva |
| Species Category | Human | Any Species, Bovine, Canine, Chicken, Hamster, Human, Maize, Mouse, Ovine, Porcine, Rice, Soybean, Tomato | Human, Other |
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