Sequencing Library Preparation Methods
Sequencing Method Explorer
Use this interactive tool to explore experimental NGS library prep methods compiled from the scientific literature. The tool includes a subset of methods from the review articles and posters below. New methods will be added as they become available.
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Sequencing Method Posters
View illustrated schematics of DNA, RNA, and single-cell library preparation techniques that are compatible with SBS technology. Also see brief diagrams of Illumina library preparation kit protocols.
For All You Seq—DNA
This poster includes NGS methods for analyzing:
- DNA variants
- Low levels of DNA
- Epigenetics
- DNA–protein interactions
- Protein–protein interactions
For All You Seq—RNA
This poster includes a comprehensive set of NGS methods for analyzing:
- RNA transcription
- RNA–protein interactions
- Low levels of RNA
- RNA modifications
- RNA structure
For All You Seq—Single-Cell
This poster includes a comprehensive set of NGS-based single-cell analysis methods for:
- Low-level RNA detection
- Low-level DNA detection
- Integrated DNA and RNA analysis
Compare a Broad Range of Methods
Explore the world's largest collection of NGS library preparation techniques. These methods, compiled from the scientific literature, demonstrate the wide range of scientific questions you can address with Illumina sequencing by synthesis (SBS) technology.
These comprehensive review articles describe the various NGS methods, with pros and cons, publication summaries, and references.
Read DNA Methods ReviewRead RNA Methods ReviewFeatured Method
scTrio-Seq
scTrio-Seq can analyze genomic CNVs, the DNA methylome, and the transcriptome of an individual mammalian cell simultaneousl. This approach is an extension of previous methods, such as scMT-seq.
Pros
- Accurately analyzes the mechanism by which the transcriptome, genome, and DNA methylome regulate each other
- CNVs can be identified reliably using scRRBS data
Cons
- Lower transcriptome coverage than scMT-seq1
- Results in 3'-biased transcriptome
SBS Technology
Learn more about the advantages of sequencing by synthesis, how it works, and the many ways you can leverage sequencing technology to help answer your research questions.
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