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Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
These sequencing reagent kits offer a simplified workflow and high data quality for in vitro diagnostic testing on the NextSeq550 Dx Instrument.
This product is only available in select locations. Please select the location where you would like this product to be shipped to see availability.
NextSeq 550Dx Reagent Kits contain reagents and consumables for supporting high-throughput sequencing of libraries for in vitro diagnostic testing.
NextSeq 550Dx Reagents feature increased shelf-life, single-lot shipment, and advance change notification, and include a certificate of analysis for each shipment. The kits are developed under design control principles, manufactured under cGMP, and verified to ensure consistent assay performance.
Each kit provides all components needed for sequencing on the platform in three ready-to-use cartridges (reagent, flow cell, and buffer). The kit offers simplified cartridge loading with all required reagents already in the cartridge. Simply load user-prepared library pools directly into the NextSeq 550Dx reagent cartridge and the instrument will automatically perform all steps for template amplification and sequencing without user intervention.
The Illumina NextSeq 550Dx High Output Reagent Kit v2.5 (300 cycles) is a set of reagents and consumables intended for sequencing of sample libraries when used with validated assays. The kit is intended for use with the NextSeq 550Dx instrument and analytical software.
The Illumina NextSeq 550Dx High Output Reagent Kit v2.5 (75 cycles) is a set of reagents and consumables intended for sequencing of sample libraries when used with validated assays. The kit is intended for use with the NextSeq 550Dx instrument and analytical software.
The NextSeq 550Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used for in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx instrument is to be used with registered and listed, cleared or approved, IVD reagents and analytical software.
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is to be used with specific registered, certified or approved IVD reagents and analytical software.
Illumina DNA Prep with Enrichment Dx is a set of reagents and consumables used to prepare sample libraries from DNA extracted from peripheral whole blood and formalin-fixed, paraffin-embedded tissue. User-supplied probe panels are required for the preparation of libraries targeting specific genomic regions of interest. The generated sample libraries are intended for use on Illumina sequencing systems.
Illumina DNA Prep with Enrichment Dx is a set of reagents and consumables used to prepare sample libraries from genomic DNA derived from human cells and tissue. User-supplied probe panels are required for the preparation of libraries targeting specific genomic regions of interest. The generated sample libraries are intended for use on Illumina sequencing systems.
For In Vitro Diagnostic Use
Contact an Illumina representative for regional availability.
Hands-on time | 10 min |
---|---|
Instruments | NextSeq 550Dx Instrument |
Nucleic acid type | DNA |
Reagent type | Paired-end sequencing, Cluster generation, Sequencing by synthesis, Single-read sequencing |
Species category | Any species |
Technology | Sequencing |
Select the NextSeq 550Dx Reagent Kit that matches the number of cycles that supports your read length needs. Intended for use on the NextSeq 550Dx Instrument.
Also requires use of a validated library preparation solution:
The optional DRAGEN On-Premise Server for NextSeq 550Dx provides sequencing run setup, FASTQ file, and germline and somatic secondary analysis for libraries generated by IDPE Dx.
The NextSeq 550Dx Reagent Kit is used by clinical laboratories for in vitro diagnostic testing and assay development on the NextSeq 550Dx Instrument.
Illumina Manager – IDPE Dx app
Advancements in our understanding of genetics have the potential to change the practice of medicine and enable genomics-based health care.
Illumina offers next-generation sequencing-based molecular diagnostic options for clinical laboratories.
A dedicated support section is not currently available for this product
NextSeq 550Dx High Output Reagent Kit v2.5 (300 cycles) IVD
20028871
Includes one high output flow cell, one buffer cartridge, and one reagent cartridge containing clustering and sequencing reagents to support a 300-cycle run on the NextSeq 550Dx System. All products registered for In Vitro Diagnostic sequencing.
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NextSeq 550Dx High Output Reagent Kit v2.5 (75 cycles) IVD
20028870
Includes one high output flow cell, one buffer cartridge, and one reagent cartridge containing clustering and sequencing reagents to support a 75-cycle run on the NextSeq 550Dx System. All products registered for In Vitro Diagnostic sequencing.
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Each kit includes three ready-to-use cartridges containing all the components needed for sequencing on the platform (reagent, flow cell, buffers).
TruSight Oncology Comprehensive (EU) and VeriSeq NIPT Solution v2 are validated for use with NextSeq 550Dx Reagents on the NextSeq 550Dx Instrument.
Illumina DNA Prep with Enrichment Dx can be used with user-supplied probe panels to generate DNA libraries, which can be sequenced in the diagnostic mode of the instrument using NextSeq 550Dx reagent kits. The onboard Local Run Manager Generate FASTQ Dx module provides sequencing run setup and FASTQ files. The optional DRAGEN server for NextSeq 550Dx provides sequencing run setup, FASTQ files, and germline and somatic secondary analysis.
NextSeq 550Dx High-Output Reagent Kit v2.5 (300 Cycles) specifications: read length: 2 × 150 bp; clusters passing filter (per flow cell): 400 million; max output: 120 Gb; run time: < 25 hr; data quality (Q30): > 75%.
NextSeq 550Dx High-Output Reagent Kit v2.5 (75 Cycles) specifications: read length: 1 × 75 bp; clusters passing filter (per flow cell): 400 million; max output: 30 Gb; run time: < 11 hr; data quality (Q30): > 80%.
The NextSeq 550Dx Instrument is an FDA-regulated and CE-marked IVD high-throughput sequencing system that provides both diagnostic and research modes. The NextSeq 550 System is for research use only (RUO). In vitro diagnostic (IVD) testing is only supported on the NextSeq 550Dx Instrument. NextSeq 550Dx reagent kits are compatible with both diagnostic and research mode on the Dx instrument but are not compatible with the RUO system.
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