Specifications for the MiSeqDx system

The MiSeqDx instrument has validated performance characteristics for accurate and reliable data

MiSeqDx Specifications

Maximum Read Length Up to 2 × 300 bp
(Refer to package insert for assay-dependent specifications)
Output (2 x 150 bp run) ≥ 5 Gb
Reads passing filters ≥ 15 million
Accuracy-Germline* >99.9% Overall Percent Agreement to Reference Results
Accuracy-Somatic* 100% Overall Percent Agreement to Reference Results
Reproducibility-Germline** 99.88% Overall Percent Agreement to Reference Results
Reproducibility-Somatic*** 99.6% Percent Expected Call for mutant samples
Q30 score (at read length of 2 × 150 bp) ≥ 80%
Power Requirements 100–240V AC at 50/60Hz, 10A, 400W
RFID Radio Frequency 13.56 MHz
RFID Power 100 mW
Throughput 1–96 samples/run, depending upon assay
LED 530, 660 nm

*Results based on a representative TruSeq Amplicon-based assay designed to query a variety of genes covering 12,588 bases across 23 different chromosomes using 150 amplicons

**Results based on Cystic Fibrosis (CF-139) Assay

***Results based on a representative 2-gene TruSeq Amplicon-based assay

Temperature Transportation and Storage: -10°C to 40°C (14°F to 104°F) Operating Conditions: 19°C to 25°C (66°F to 77°F)
Humidity Transportation and Storage: Non-condensing humidity Operating Conditions: 30–75% relative humidity (non-condensing)
W×D×H 68.6 cm × 56.5 cm × 52.3 cm (27.0 in × 22.2 in × 20.6 in)
Weight 54.5 kg (120 lbs)
Crated Weight 90.9 kg (200 lbs)
Base Unit Intel Core i7-7700 2.9 GHz CPU
Memory 16 GB RAM
Hard Drive 2 × 1 TB
Operating System Windows 10

Specifications shown here are for IVD use. View research use only specs, applicable only when running MiSeqDx in Research mode.

MiSeqDx System Specification Sheet

MiSeqDx System Specification Sheet

Designed specifically for the clinical laboratory environment, the MiSeqDx instrument offers a small, approximately 4 square feet (0.3 square meters) footprint, an easy to follow workflow, and data output tailored to the needs of clinical labs.

View Specification Sheet
Sequencing by Synthesis (SBS) Technology

The MiSeqDx System harnesses proven Illumina SBS technology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.

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Paired-End Sequencing Technology

Paired-end sequencing enables both ends of the DNA fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads over repetitive regions more precisely. This results in much better alignment of the reads, especially across difficult-to-sequence, repetitive regions of the genome.

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