iSeq 100 Sequencing System applications and methods

Multi-application flexibility

Flexible read lengths allow an easy transition from small-scale DNA and RNA sequencing projects

Key applications and methods

Targeted DNA sequencing
Bacterial sequencing
Viral sequencing

Targeted DNA sequencing allows researchers to focus on a select set of genes or relevant gene regions using fixed panels of expert-selected content or custom, user-defined gene panels. By focusing the power of next-generation sequencing (NGS), targeted sequencing enables cost-effective and systematic variant detection with high accuracy and reproducibility.

Read application note: amplicon cancer panels

Prep

AmpliSeq for Illumina Cancer HotSpot Panel v2

Targeted panel investigating hotspot regions of 50 genes with known associations to cancer.

AmpliSeq for Illumina Focus Panel

Targeted DNA and RNA panel investigating 52 genes with known relevance to solid tumors.

AmpliSeq for Illumina BRCA Panel

Targeted panel investigating somatic and germline variants in BRCA1 and BRCA2.

AmpliSeq for Illumina Immune Response Panel

Targeted RNA expression panel investigating 395 genes involved in tumor-immune system interactions.

AmpliSeq for Illumina Custom DNA Panel

Targeted custom panel optimized for specific targets or genomic content of interest.

Sequence

iSeq 100 Reagents

Reagents include a flow cell and prefilled, ready-to-use cartridges containing clustering and sequencing reagents to support cycling runs on the iSeq 100 System.

Analyze

BaseSpace Sequence Hub

The Illumina genomics cloud-computing environment for NGS data analysis and management.

Find targeted DNA sequencing education

Bacterial sequencing enables comprehensive characterization of bacterial genomes, including antibiotic resistance, molecular epidemiology, and virulence. Using NGS, sequencing of bacterial genomes can be done quickly while producing highly accurate data.

Read surveillance application note

Library prep

Illumina DNA Prep

Fast, integrated workflow for a wide range of applications, including sequencing whole human genomes, amplicons, plasmids, and microbial genomes.

Sequence

iSeq 100 Reagents

Reagents include a flow cell and prefilled, ready-to-use cartridges containing clustering and sequencing reagents to support cycling runs on the iSeq 100 System.

Analyze

BaseSpace Sequence Hub

The Illumina genomics cloud-computing environment for NGS data analysis and management.

Find bacterial sequencing education

Viral sequencing is a fast and effective tool for characterizing viral agents, studying viral variability, and identifying genetic markers associated with virulence.

Read application note: pan-viral sequencing

Read customer story: Ebola virus sequencing

Library prep

Illumina DNA Prep

Fast, integrated workflow for a wide range of applications, including sequencing whole human genomes, amplicons, plasmids, and microbial genomes.

Viral Surveillance Panel

Targeted RNA panel investigating 66 viruses that are of high risk to public health.

Sequence

iSeq 100 Reagents

Reagents include a flow cell and prefilled, ready-to-use cartridges containing clustering and sequencing reagents to support cycling runs on the iSeq 100 System.

Analyze

BaseSpace Sequence Hub

The Illumina genomics cloud-computing environment for NGS data analysis and management.

Find viral sequencing education

More applications and methods

16S metagenomic sequencing

Sequence the 16S ribosomal RNA (rRNA) gene with a culture-free method to identify and compare bacteria from complex, difficult-to-study microbiomes or environments.

Learn more about 16S metagenomics

Read application note

Quality control

Perform quality control (QC) applications for bioproduction studies or assess the quality of a sequencing library before committing it to a full-scale run.

Read application note

Read customer interview

QC and rebalancing of single-cell libraries

Evaluate the quality and enable accurate rebalancing of multiplexed single-cell gene expression libraries before committing to a full-scale run.

Read application note

Small whole-genome sequencing

Sequence the entire genome of a bacterium, virus, or other microbe for applications in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.

Learn more about small WGS

Targeted gene sequencing

Select and sequence specific genes or genomic regions of interest using predesigned or custom designed panels for gene profiling studies.

Learn more about targeted gene sequencing

Read application note

Targeted RNA sequencing

Select and sequence specific transcripts of interest for gene expression profiling studies.

Learn more about targeted RNA-Seq

How others use this instrument

See what’s possible with the iSeq 100 System

Environmental DNA (eDNA) sequencing offers a powerful lens to view changes in biodiversity

Researchers at Curtin University in Perth, Australia use the iSeq 100 System to analyze eDNA samples and monitor ecosystem changes over time.

The time is now for microbiome studies

Whole-genome shotgun sequencing and transcriptomics provide researchers and pharmaceutical companies with data to refine drug discovery and development.

For every lab, everywhere

NextSeq 1000 and NextSeq 2000 Reagents

NGS Workflow Finder

DRAGEN v4.3 now available on-premises and on-cloud

Illumina Proactive Instrument Performance Service

Do more, faster than ever

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Next-generation sequencing for beginners

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

Celebrate DNA Day with Your Students

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

iSeq 100 Sequencing System applications and methods

For every lab, everywhere

Key applications and methods

Prep

Sequence

Analyze

Library prep

Sequence

Analyze

Library prep

Sequence

Analyze

Methods guide for microbial whole-genome sequencing

More applications and methods

16S metagenomic sequencing

Quality control

QC and rebalancing of single-cell libraries