Infinium Custom Methylation Kit

Fully customizable methylation microarray for large, targeted epigenetic applications.

24 samples per array

Number of samples

See full details in the specifications table

Overview

The Infinium Custom Methylation Kit contains the Infinium HTS iSelect Methyl Custom BeadChip plus reagents to enable researchers and commercial users to develop groundbreaking assays for critical epigenetic research applications.

High-throughput workflow

The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.

High-throughput workflow

The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.

High-throughput workflow

The Infinium HTS iSelect Methyl Custom BeadChip is offered in a 24-sample format with updated Infinium automation protocols and an automated workflow for bisulfite conversion. These features improve workflow efficiency, decrease sample processing costs, and allow users to scale their operations up to hundreds of thousands of samples per year.

Key features of the Infinium Custom Methylation Kit

  • Customizable content supports up to 100K markers

  • Accurate quantitative measurement of DNA methylation at the single-CpG-level

  • Rapid turnaround time with optional automated bisulfite conversion


Specifications


Required Products

Requires purchase of the Infinium HTS iSelect Methyl Custom BeadChip-24 kit. Each kit contains 2 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 DNA samples.

Requires an iScan Sequencing System.

Optional introductory course for 3 days of hands-on instruction covering all essential steps in the Infinium protocol, including sample and BeadChip preparation, sample scanning, and primary data evaluation and analysis using array analysis software is available for purchase.

/ Results

Applications

The Infinium Custom Methylation Kit allows users to create custom high-throughput assays to measure DNA methylation in targeted regions of the genome for commercial and academic epigenetic applications, including common disease research, cancer research, epigenetic aging, and nutriepigenomics.

Example workflow

1
Method
2
Scan
3
Analyze

Related applications and methods

Documentation

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Figures

Infinium I and II assays allow for broader coverage of CpG sites

Illumina methylation BeadChips employ both Infinium I and Infinium II assays, enhancing the overall breadth of coverage. (A) Infinium I probe designs use 2 bead types per CpG locus, 1 each for the methylated and unmethylated states. (B) Infinium II probe designs use 1 bead type for both states.

Design and manufacturing process

Researchers work with Illumina technical design and customer service teams to refine the BeadChip marker content and coordinate the ordering and production process.

  1. Zhou L, Ng HK, Drautz-Moses DI, et al. Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing. Sci Rep. 2019;9(1):10383. Published 2019 Jul 17. doi:10.1038/s41598-019- 46875-5

Resources

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Discover the Flexible, Scalable Infinium Workflow

Infinium Methyl Custom-24 Kit (48 Spl)

20042146

Infinium HTS iSelect Methyl Custom Beadchip, 48 samples

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Infinium® Assay: An Introduction – Customer Site

20015273

Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software.

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FAQs

 The Infinium HTS iSelect Custom BeadChip includes the standard SNP controls available on the Methylation EPIC BeadChip.

The GenomeStudio Methylation Module extracts beta values and provides sample clustering analysis for Infinium HTS iSelect Methyl Custom BeadChip data. The BeadArray Controls Reporter software tool is not supported at this time.

Notably, there are many freeware applications in Bioconductor, such as SeSAMe, that provide enhanced normalization and visualization options. Illumina does not support these freeware solutions directly.

No, Illumina LIMS does not support Infinium HTS iSelect Methyl Custom BeadChips at this time.

No, FFPE samples are not currently supported.

The Zymo Research EZ-96 Methylation-Lightning MagPrep Kit in manual and automated formats is supported for use upstream to Infinium Methylation assays. Review results in this technical note for more details. 
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