Illumina Technical Support introductory presentation on the Illumina Single Cell RNA Prep Kit, formerly known as PIPseq. This webinar is geared towards new to single cell users and intermediate users with a basic knowledge of next generation sequencing. The following topics will be covered: • Single Cell Experiment Best Practices • Sample Preparation Best Practices for Illumina Single Cell • Supplemental Enrichment Amplification (SEA) kit Applications Subscribe to the Illumina video channel http://www.youtube.com/subscription_center?add_user=IlluminaInc A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View Illumina webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View Illumina support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: https://www.linkedin.com/company/illumina X: https://x.com/illumina Instagram: https://www.instagram.com/illuminainc/ Facebook: https://www.facebook.com/illuminainc TikTok: https://www.tiktok.com/@illuminainc 0:00 Introduction 05:07 Single Cell Experiment Best Practices 05:13 Experimental Design 10:10 Equipment Preparation 11:49 Single Cell Workflow Overview 14:50 cDNA QC 15:41 Library Preparation Workflow 17:47 Library Prep QC 19:54 Sequencing Recommendations 23:42 Molecular Counting Strategy 26:38 Sample Prep Best Practices for Illumina Single Cell 27:19 Cells or Nuclei 35:25 Enrichment Strategies 36:21 Flow Sorting Recommendations 43:45 Supplemental Enrichment and Amplification Kit #IlluminaSingleCellRNAPrep #T2Workflow #T10Workflow #T20Workflow #T100Workflow #illumina