Nucleic acid QC with the Agilent Fragment Analyzer system
The Fragment Analyzer system accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing.
Minimize errors, reduce hands-on-time, and enable higher throughput with automation options across Illumina library prep products
Automated liquid-handling systems help labs achieve and maintain consistent results while driving efficiency. NGS library prep automation also allows for the rapid scaling of throughput while reducing hands-on time.
Illumina partners with leading vendors to provide automated protocols for Illumina library prep kits, combining our partners’ automation knowledge with our library prep and sequencing expertise to build an NGS solution that works for you.
This guide walks you through the range of automation tools available for NGS library prep and helps you find a solution tailored for your application, throughput, and budget needs. Learn about top considerations for building your automated library prep protocol.
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Illumina Qualified methods are developed, tested, distributed, owned, and supported by our partners. Illumina reviews data to ensure method performance and data quality.
Vendor-developed methods are those that have been developed by an automation partner, but have not undergone data review by Illumina.
Product Brand | Illumina Qualified |
Vendor-developed |
AmpliSeq for IlluminaAmpliSeq chemistrya offers a highly multiplexed PCR-based workflow for a few to hundreds of targets in a single run. |
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COVIDSeq Assay (96 samples)This low- to mid-throughput NGS assay enables labs of any size to identify and track the emergence and prevalence of novel SARS-CoV-2 variants. |
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COVIDSeq Test (RUO Version)This high-throughput NGS assay enables labs to detect SARS-CoV-2 mutations to identify and track the emergence and prevalence of novel variants. |
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Illumina DNA PCR-Free PrepAn efficient, fast, and integrated library preparation workflow that yields highly accurate data for sensitive sequencing applications. |
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Illumina DNA PrepA fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications. |
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Illumina DNA Prep with EnrichmentA fast, integrated workflow that provides sequencing-ready DNA libraries for a wide range of targeted sequencing applications. |
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Illumina RNA Prep with EnrichmentA fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities. |
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Illumina Stranded mRNA PrepA rapid, cost-effective workflow for accurate, unbiased detection of the protein-coding transcriptome with precise measurement of strand information. |
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Illumina Stranded Total RNAStreamlined RNA-Seq solution for clear and comprehensive analysis across the coding and noncoding transcriptome with exceptional study flexibility. |
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Nextera XT DNAA 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement. |
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TruSight Oncology 500Assay enabling comprehensive genomic profiling with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI, and HRD*) from FFPE tissue. *HRD is only available with the addition of the TruSight Oncology 500 HRD kit to TruSight Oncology 500. Not available in Japan. |
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TruSight Oncology 500 High-ThroughputAssay enabling comprehensive genomic profiling with a large pan-cancer panel covering all major variant classes plus gene signatures (TMB, MSI) from FFPE tissue, with added scalability. |
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TruSeq DNA NanoGenerate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA. |
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TruSeq DNA PCR-FreeSimple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes. |
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TruSeq Stranded mRNAPrepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information. |
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TruSeq Stranded Total RNAA robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for various species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue. |
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TruSight Tumor 15Focused NGS panel to assess 15 commonly mutated genes in solid tumors with a simple and rapid workflow. |
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TruSight Tumor 170Comprehensive NGS assay targeting 170 genes associated with solid tumors. |
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Track samples, manage your runs, and launch into production with preset laboratory information management system (LIMS) protocols. Leverage a compatible robotic liquid handler to prepare libraries for rapid high-throughput sequencing and data analysis.
Laboratory information management system designed to track samples and manage workflows for an optimized and efficient lab.
Sequencing systems delivering up to 16 Tb of output, allowing sequencing of 128 human genomes at 30× coverage in a single run.
Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
Illumina DRAGEN secondary analysis
Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing data.
A comprehensive research software platform to support tertiary analysis clinical research workflows.
The Fragment Analyzer system accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing.
This streamlined automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.
COVIDSeq Assay and COVIDSeq Test workflows are compatible with a range of automation solutions and configurable for batch sizes from 8–96 samples.
Contact us to learn more about automation partners and Illumina Qualified methods.
Your email address is never shared with third parties.