Synthetic Biology

To confirm your constructs, you can use next-generation sequencing (NGS) to provide a high-throughput, scalable, and accurate approach to validate your targets, whether it is DNA, RNA, or both. To learn more about NGS, visit our page below:

• Introduction to NGS

Confirming your constructs through next-generation sequencing is an essential step in validating the accuracy of edited genes. However, it is important to consider other aspects of your biological system. Examples include analyzing off-target effects, confirming deletions, and determining how edited genes are modified. For information related to genome editing, such as using CRISPR/Cas9, visit our page below to learn how NGS can optimize your synthetic biology experiments:

• CRISPR Genome Editing

Generally, your NGS workflow's main steps include library preparation, sequencing, and data analysis. Illumina has helpful guides and pages for these steps, as well as an overview of the workflow steps here:

• NGS Workflow Overview
• Preparing DNA Libraries
• Sequencing Overview
• Analyzing NGS Data

Illumina offers flexible NGS platforms with exceptional quality, accuracy, and speed for various throughput capabilities. Visit our Sequencing Platform page to find sequencing platforms that best suit your needs.

There are many considerations in estimating the cost of using NGS for synthetic biology. Illumina offers a dedicated page that provides a cost breakdown for a wide array of NGS applications to suit your budget, including flexible purchasing options. Visit our Planning your NGS Budget page or contact us for more information to get started.