Instruments
    • Instruments

    NovaSeq X Series: optimized for comprehensive omic solutions

    Expand biological discovery with the power of high-throughput multiomics 

    Harness omic solutions with the NovaSeq X Series 

    The NovaSeq X Series delivers the scalable sequencing power and flexibility to support a broad range of methods for interrogating the genome, epigenome, transcriptome, and proteome. Engineered to output data with the highest integrity, the NovaSeq X Series makes even the most data-intensive research accessible with industry leading speed and throughput.

    Unleash discovery power across multiple omes with innovations designed for the NovaSeq X Series

    Flexibility

    Suitable for both small data sets and large-scale multiomic studies, supporting multiple sample types, e.g. bulk, single-cell, and spatial. Preconfigured and customizable workflows provide flexibility to meet different user needs.

    Scalability

    Multiple cycle kits support a wide range of scalable options. The NovaSeq X Plus System and 1.5B, 10B, and 25B flow cells enable data-intensive multiomic studies at a broader and deeper scale than ever before.

    Hongyu Gao, Phd


    We have been combining samples from multiple projects and users on the same large run on the NovaSeq X Series with a 10B or 25B flow cell.

    Hongyu Gao, Phd
 Associate Scientist
 Indiana University Genomics Core
    Read flyer: Make NGS easier and more affordable

    Efficiency

    Reduced touch points and steps allow faster and easier workflows from sample preparation to data interpretation. Individually addressable lanes help tailor sequencing data per library.

    Cost

    Process more samples and access more omes with up to 3× higher accuracy.1 The 25B flow cell increases the number of samples by up to 2.5× for the same budget.2

    Elizabeth Pritchett, PhD

    This 25B flow cell is a great example of the diverse library types we are able to sequence in an efficient way.

    Elizabeth Pritchett, PhD Operations Director Rochester Genomics Center
    Read flyer: Enable more sequencing for more labs
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    The NovaSeq X Series: Achieve single-cell discoveries powered by 25 billion reads

    The time is now for multiomics to address complex biological questions. Watch the video to discover how scientists are gaining an advantage in their sample-to-discovery journey through the power of the NovaSeq X Series.

    Technical note spotlight

    Perturb-Seq on the NovaSeq X 25B flow cell

    Learn how you can achieve high-throughput, genome-scale Perturb-Seq experiments with NovaSeq X 25B flow cells. In this technical note, in collaboration with Single Cell Discoveries, we demonstrate a large-scale CRISPR interference workflow. 

    Download tech note

    Integrated multiomic workflows for the NovaSeq X Series

    1. Library prep solutions

    A broad portfolio of high-throughput Illumina and third-party solutions to interrogate the genome, epigenome, trancriptome, and proteome.

    2. Sequencing with NovaSeq X Series

    The NovaSeq X and NovaSeq X Plus Sequencing Systems provide exceptional throughput and accuracy for your data-intensive applications at scale.

    3. Illumina Connected Analytics

    Experience the power of our secure and flexible bioinformatics platform, designed to operationalize informatics workflows and drive groundbreaking scientific insights.

    • Technical note PDF 1 MB 2 versions

    Omic methods and workflows with the NovaSeq X Series

    The NovaSeq X Series makes multiomics accessible to enable discoveries that were previously out of reach. Explore products and reagents, with supporting application notes, resources, and relevant analysis solutions to interpret results for omics workflows below. 

    By studying the genome, researchers can use information about the structure, function, and sequence of DNA to better understand its role in health and disease.

    Featured solutions and resources

    Illumina DNA PCR-Free Prep

    A rapid and flexible workflow for sensitive whole-genome sequencing.

    Illumina DNA Prep

    A fast, robust, and flexible workflow for a wide range of DNA input types.

    Illumina DNA Prep with Enrichment

    The fastest and most flexible targeted DNA sequencing solution from Illumina.

    Illumina Cell-Free DNA Prep with Enrichment

    A highly sensitive library preparation solution to detect low-abundance mutations from cell-free DNA samples. Learn more in our data sheet.

    Illumina Complete Long Reads

    Explore products for Illumina Complete Long Read sequencing.

    Omics data analysis and interpretation solutions

    Illumina offers a comprehensive portfolio of software solutions to analyze omic data and lead to novel insights. Explore informatic solutions capable of scaling to production-level systems such as the NovaSeq X Series to streamline data to insight with efficiency and ease.

    Software solutions

    DRAGEN secondary analysis

    Get accurate, comprehensive, and highly efficient bioinformatics with multiple deployment options, applications, and pipelines to meet your research needs.  

    BaseSpace Sequence Hub

    Integrates with Illumina sequencing systems with an intuitive interface for streamlined run setups and monitoring. Enjoy intuitive push-button secondary analysis and data sharing within a secure cloud ecosystem with expandable storage for growing laboratory needs.

    Illumina Connected Analytics

    A secure, scalable, bioinformatics platform that enables you to manage, analyze, and interpret large volumes of multiomic data. 

    Partek Flow analysis solution

    Partek Flow bioinformatics software provides a visual user interface, robust statistical algorithms, information-rich visualizations, and cutting-edge genomic tools that enable researchers of all skill levels to confidently perform data analysis. 

    Illumina Correlation Engine

    An interactive omics knowledgebase that puts private omics data in biological context with highly curated public data.

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    Illumina Connected Analytics for multiomic cancer applications

    Watch this Illumina Connected Analytics workflow demo specific to Cohorts for multiomic cancer analysis.

    Software articles and resources

    DRAGEN for single-cell genomic insights

    Read this update on the introduction of innovative pipelines for DRAGEN, including single-cell ATAC-Seq and single-cell multiomics (RNA-Seq and ATAC-Seq).

    BaseSpace Sequence Hub with 10x Genomics libraries

    Learn how sequencing runs with 10x Genomics libraries can now be directly set up on the Run Planner. The Run Planner tool guides you through the steps needed for preparing your sequencing run, such as specifying sample and index information, read length and analysis options.

    Partek bioinformatics for spatial multiomics

    Learn how to combine RNA and protein data, statistically assess relationships using correlation between gene and protein molecules, and explore differences between cell types using differential analysis comparisons for biological interpretation.

    Application note spotlight

    Cancer-specific protein patterns of single cells

    In this application note, learn how the ability to measure and visualize 80 surface proteins across 1000 single cells per sample—utilizing the Pixelator pipeline and analysis package—can enhance your understanding of immune cell behavior in cancer.

    Download app note

    Related eBooks

    The power of multiomics eBook

    See how you can use multiomics to better connect genotype to phenotype and obtain a full cellular readout not found through single omics approaches.

    Multiomic sequencing with the NovaSeq X Series eBook

    Learn how you can perform broader, deeper sequencing with the NovaSeq X Series and take on omics projects that were previously out of reach.

    Gene expression and regulation bioinformatics eBook

    Learn more about the NGS data analysis workflow and get an overview of software solutions available for analyzing gene expression and regulation data.

    Single-cell sequencing eBook

    Explore every step of the single-cell sequencing workflow and learn valuable insights to ensure experimental success.

    Additional resources

    TruSight Oncology ctDNA v2 on the NovaSeq X Series

    In this app note, we compare the performance of the NovaSeq X and NovaSeq 6000 using the TruSight Oncology ctDNA v2 CGP assay. Results demonstrate the same high level of performance with significantly reduced run times when using the NovaSeq X Series.

    Accelerate omics pattern discovery using Correlation Engine

    Read how Correlation Engine provides a curated omics knowledge base of biological interactions that is driven by the vast amount of multiomic public data and publications, along with the built-in comprehensive analytic correlation tools.

    FAQ

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    References

    1. With XLEAP-SBS Chemistry
    2. Compared to the NovaSeq 6000 S4 flow cell