Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
---|---|---|
1 × 75 bp | N/A | 25–30 Gb |
2 × 75 bp | 16.25–19.5 Gb | 50–60 Gb |
2 × 150 bp | 32.5–39 Gb | 100–120 Gb |
High-accuracy sequencing and array scanning
Tunable read length and multiple output configurations
Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
---|---|---|
1 × 75 bp | N/A | 25–30 Gb |
2 × 75 bp | 16.25–19.5 Gb | 50–60 Gb |
2 × 150 bp | 32.5–39 Gb | 100–120 Gb |
a. Based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 550 System kits are paired-end capable.
NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
---|---|
3 exomes | 1 whole human genome |
12 enrichment panels | 12 exomes |
96 amplicon panels | 16 transcriptomes |
NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit | |
---|---|---|
Single reads | ≤ 130M | ≤ 400M |
Paired-end reads | ≤ 260 M | ≤ 800 M |
Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
---|---|---|
1 × 75 bp | N/A | > 80% bases higher than Q30 |
2 × 75 bp | > 80% bases higher than Q30 at 2 × 75 bp | > 80% bases higher than Q30 |
2 × 150 bp | > 75% bases higher than Q30 at 2 × 150 bp | > 75% bases higher than Q30 |
a. A quality score is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
---|---|---|
1 × 75 bp | N/A | 11 hr |
2 × 75 bp | 15 hr | 18 hr |
2 × 150 bp | 26 hr | 29 hr |
Array | Scan time per array | Scan time per sample |
---|---|---|
Infinium MethylationEPIC BeadChip | 40 min | 5 min |
Infinium CytoSNP-850K BeadChip | 40 min | 5 min |
Sequencing by synthesis chemistry
The NextSeq 550 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
2-Channel sequencing by synthesis
The NextSeq 550 System leverages 2-channel sequencing by synthesis (SBS) for faster data generation than the original Illumina 4-channel SBS technology, with the same high data accuracy.