MiSeqDx Instrument specifications

Validated performance. Reliable results.

A powerful benchtop sequencing system that delivers exceptional data quality and reliability for IVD assay testing, development, and clinical research 

MiSeq Dx instrument render

Output per flow cell for various read lengthsa

Read length MiSeqDx Reagent Kit v3
2 x 150 bp ≥ 5 Gb

a. The MiSeqDx Instrument runs a single flow cell. Output per flow cell and output per run are the same. 

Estimated sample throughput for key applications

Sample type MiSeqDx Reagent Kit v3
Human peripheral whole blood for cystic fibrosis testing 24-96 samples per run

Reads passing filter per flow cell

Sample type MiSeqDx Reagent Kit v3
Paired-end reads ≥ 15M

Quality scoresa

Read length MiSeqDx Reagent Kit v3
2 x 150 bp ≥ 80% of bases higher than Q30

a. A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.

Run time

Read length MiSeqDx Reagent Kit v3
2 x 150 bp 24 hr

Total overall accuracy

Variant calling Accuracy
Germlinea > 99.9% overall percent agreement to reference results
Somatica 100% overall percent agreement to reference result

a. Results based on a representative TruSeq Amplicon–based assay designed to query a variety of genes covering 12,588 bases across 23 different chromosomes using 150 amplicons.

Total overall reproducibility

Variant calling Accuracy
Germlinea 99.88% overall percent agreement to reference results
Somaticb 99.6% percent expected call for mutant sample

a. Results based on TruSight Cystic Fibrosis 139-Variant Assay.

b. Results based on a representative 2-gene TruSeq Amplicon-based assay.

Instrument specifications

MiSeq Dx instrument specifications

MiSeqDx specifications

  • Light emitting diode (LED)
    Wavelengths
    520 nm, 660 nm
  • Dimensions
    W x D x H
    68.6 cm × 56.5 cm × 52.3 cm
    (27.0 in × 22.2 in × 20.6 in)
  • Dry weight
    54.5 kg (120 lb)
  • Crated weight
    90.9 kg (200 lb)

Specifications shown here are for IVD use. View research use only specifications, applicable when running the MiSeqDx Instrument in Research Mode. 

Key technologies

Intended use for the MiSeqDx Instrument
United States:

The MiSeqDx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood, formalin-fixed, paraffin-embedded (FFPE) tissue, or embryonic tissue, when used with in vitro diagnostic (IVD) assays performed on the instrument. The MiSeqDx Instrument is not intended for whole genome or de novo sequencing. The MiSeqDx Instrument is to be used with registered and listed, cleared, or approved IVD reagents and analytical software.

European Union/other:

The MiSeqDx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used with in vitro diagnostic (IVD) assays performed on the instrument. The MiSeqDx Instrument is not intended for whole genome or de novo sequencing. The MiSeqDx Instrument is to be used with registered and listed, cleared, or approved IVD reagents and analytical software.