Depletion of ribosomal RNA (rRNA) prior to RNA sequencing (RNA-Seq) allows researchers to focus on analyzing high-value portions of the transcriptome. Illumina rRNA removal solutions set the standard for rRNA and globin mRNA depletion for RNA-Seq library preparation.
The rRNA depletion process:
| Kit | Kit Description | RNA Sample Types | Removes Euk. Cytoplasmic rRNA | Removes Mitochondrial rRNA | Removes Bacterial rRNA | Removes Chloroplast rRNA | Removes Globin mRNA | Replaces Discontinued Kit |
|---|---|---|---|---|---|---|---|---|
| Illumina Stranded Total RNA Prep with Ribo-Zero Plus | Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility. | Human and other animal species; FFPE RNA | ✓ | ✓ | ✓ | ✓ | Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) |
|
| Human/mouse/rat epidemiology (eg, virus-infected) and microbiome samples |
✓ | ✓ | ✓ | ✓ | Ribo-Zero Gold rRNA Removal Kit (Epidemiology) | |||
| Bacteria (mixed populations of gram-negative and gram-positive bacteria) |
✓ | Ribo-Zero rRNA Removal Kit (Bacteria) | ||||||
| Gram-negative bacteria (E. coli) |
✓ | Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) | ||||||
| Gram-positive bacteria (Bacillus subtilis) | ✓ | Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) | ||||||
| Mammalian blood RNA | ✓ | ✓ | ✓ | ✓ | Globin-Zero Gold Kit | |||
| TruSeq Stranded Total RNA with Ribo-Zero Plant | Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. | Plant leaf tissue |
✓ | ✓ | ✓ | Ribo-Zero rRNA Removal Kit (Plant Leaf) | ||
| Plant seed or root tissue | ✓ | ✓ | ✓ | Ribo-Zero rRNA Removal Kit (Plant Seed/Root) | ||||
| Custom solution | Not currently available | Yeast | ✓ | ✓ | Ribo-Zero rRNA Removal Kit (Yeast) | |||
| TruSeq Stranded Total RNA Gold | A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue. | Human and other animal species and FFPE RNA | ✓ | ✓ | ||||
| TruSeq Stranded Total RNA with Ribo-Zero Globin | Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step. | Mammalian blood RNA | ✓ | ✓ | ✓ |