Small genome sequencing (≤ 5 Mb) involves sequencing the entire genome of a bacterium, virus, or other microbe, and then comparing the sequence to a known reference. Sequencing small microbial genomes can be useful for food testing in public health, infectious disease surveillance, molecular epidemiology studies, and environmental metagenomics.
See a detailed view of the smallest organisms with this seamless workflow solution.View Recommended Workflow
Unlike traditional approaches, small whole-genome sequencing (WGS) studies using next-generation sequencing (NGS) do not rely on labor-intensive cloning steps. NGS also enables biologists to sequence hundreds of organisms simultaneously via multiplexing. NGS can identify low-frequency variants, genomic rearrangements, and other genetic changes that might be missed or are too costly to identify using other methods. For small genomes, DNA libraries can be prepared, sequenced, and analyzed in as little as 2 days.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Speed and simplicity for targeted and small genome sequencing applications, processing up to 384 small genomes per run.
Learn how Joseph Petrosino, PhD uses whole-genome shotgun sequencing to obtain functional gene pathway and strain-based information in microbiome studies. These methods provide researchers and pharmaceutical companies with data to refine drug discovery and development.Read Article
Illumina NGS is uniquely positioned in an infectious disease surveillance and outbreak model. Compare NGS methods and find solutions to detect and characterize SARS-CoV2, track transmission routes, study co-infection, and investigate viral evolution.Learn More
Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted NGS technology, generating approximately 90% of global sequencing data.*
In addition to our industry-leading data quality, Illumina offers integrated workflows that simplify small genome sequencing, from library preparation to data analysis.
Sequence and assemble genomes of novel organisms, finish genomes of known organisms, or compare genomes across multiple samples. Learn more about microbial whole-genome sequencing.
Comprehensively analyze all genes in all organisms present in a given complex sample. Detect very low abundance microbes that may be missed or are too expensive to identify using other methods. Learn more about shotgun metagenomic sequencing.
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*Data calculations on file. Illumina, Inc., 2015