Illumina Ribo-Zero Plus rRNA Depletion Kit

A standalone kit allowing for ribosomal RNA and human globin mRNA removal in human, mouse, rat, and bacterial samples.

Overview

Reagents provided in the Illumina Ribo-Zero Plus rRNA Depletion Kit remove abundant RNA, such as ribosomal RNA and human globin transcripts, using enzymatic depletion. The remaining RNA is then converted into sequencing-ready libraries for Illumina sequencing systems using any stranded RNA prep.

Illumina Ribo-Zero Plus rRNA Depletion

Sample rRNAs Targeted
Human cytoplasmic rRNAs 28S, 18S, 5.8S, 5S
Human mitochondrial rRNAs 12S, 16S
Human beta globin transcripts HBA1, HBA2, HBB, HBG1, HBG2
Mouse & Rat rRNA 16S, 28S
Gram(-) Bacterial rRNAs E. coli: 5S, 16S, 23S
Gram(+) Bacterial rRNAs Bacillus subtilis: 5S, 16S, 23S

Specifications

Applications

The Illumina Ribo-Zero Plus rRNA Depletion Kit is a streamlined RNA-to-analysis solution for sequencing high-value, informative portions of the transcriptome.


Related applications and methods

Documentation

Product literature

Figures

Multispecies ribodepletion

Ribo-Zero Plus rRNA Depletion Kit effectively depletes rRNA levels in a variety of species. Comparison data between Illumina Stranded Total RNA Prep with Ribo-Zero Plus and TruSeq Stranded Total RNA paired with Ribo-Zero Gold or Ribo-Zero Bacteria (B. subtilis data not shown).

Effective globin mRNA depletion

Globin mRNA depletion from human peripheral blood leukocytes with Illumina Stranded Total RNA Prep with Ribo-Zero Plus using different RNA inputs.

Illumina® Ribo-Zero Plus rRNA Depletion Kit (16 Samples)

20040526

Includes Ribo-Zero Plus depletion module for depletion of ribosomal RNA from human, mouse, rat, and bacteria and globin transcripts for 96 samples. Purchase purification beads, library prep reagents and index adapters separately. For complete kit, including library prep reagents, order the Illumina Stranded Total RNA with Ribo-Zero Plus kit.

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Illumina Ribo-Zero plus rRNA Depletion Kit (96 Samples)

20037135

Includes Ribo-Zero Plus depletion module for depletion of ribosomal RNA from human, mouse, rat, and bacteria and globin transcripts for 96 samples. Purchase purification beads, library prep reagents and index adapters separately. For complete kit, including library prep reagents, order the Illumina Stranded Total RNA with Ribo-Zero Plus kit.

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FAQs

The Illumina Ribo-Zero Plus rRNA Depletion Kit depletes globin mRNAs from human, mouse, and rat blood samples. Use of this kit with other organisms has not been tested and is not supported.

There is an option to design custom supplemental spike-in probes for species not covered in the standard module. Please consult your local Sequencing Specialist to learn more about this option.

The kit removes a mix target of cytoplasmic rRNA, mitochondrial rRNA, and globin mRNA.

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