| Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
|---|---|---|
| 1 × 75 bp | N/A | 25–30 Gb |
| 2 × 75 bp | 16.25–19.5 Gb | 50–60 Gb |
| 2 × 150 bp | 32.5–39 Gb | 100–120 Gb |
NextSeq 550 Sequencing System specifications
High-accuracy sequencing and array scanning
Tunable read length and multiple output configurations
Output per flow cell for various read lengthsa
a. Based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 550 System kits are paired-end capable.
Estimated sample throughput for key applications
| NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
|---|---|
| 3 exomes | 1 whole human genome |
| 12 enrichment panels | 12 exomes |
| 96 amplicon panels | 16 transcriptomes |
Reads passing filter per flow cell
| NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit | |
|---|---|---|
| Single reads | ≤ 130M | ≤ 400M |
| Paired-end reads | ≤ 260 M | ≤ 800 M |
Quality scoresa
| Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
|---|---|---|
| 1 × 75 bp | N/A | > 80% bases higher than Q30 |
| 2 × 75 bp | > 80% bases higher than Q30 at 2 × 75 bp | > 80% bases higher than Q30 |
| 2 × 150 bp | > 75% bases higher than Q30 at 2 × 150 bp | > 75% bases higher than Q30 |
a. A quality score is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
Run time
| Read length | NextSeq 550 System Mid-Output Kit | NextSeq 550 System High-Output Kit |
|---|---|---|
| 1 × 75 bp | N/A | 11 hr |
| 2 × 75 bp | 15 hr | 18 hr |
| 2 × 150 bp | 26 hr | 29 hr |
Array scanning parameters
| Array | Scan time per array | Scan time per sample |
|---|---|---|
| Infinium MethylationEPIC BeadChip | 40 min | 5 min |
| Infinium CytoSNP-850K BeadChip | 40 min | 5 min |
Instrument specifications
-
Light emitting diode (LED)
Wavelengths
(laser diode 780 nm, Class IIIb)Green 510-525 nm, red 645-655 nm
-
Dimensions
W × D × H53.3 cm × 63.5 cm × 58.4 cm
(21.0 in × 25.0 in × 23.0 in) -
Weight
83 kg (183 lb)
-
Crated weight
151.5 kg (334 lb)
Key technologies
Sequencing by synthesis chemistry
The NextSeq 550 System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
2-Channel sequencing by synthesis
The NextSeq 550 System leverages 2-channel sequencing by synthesis (SBS) for faster data generation than the original Illumina 4-channel SBS technology, with the same high data accuracy.