NGS Tutorials

NGS tutorials

Educational resources and online tutorials

These next-generation sequencing (NGS) tutorials are designed to help you understand key concepts in NGS. With videos, online training, and technical bulletins, we’ll guide you through tips and best practices for library prep, sequencing, and data analysis.

Next-generation sequencing tutorials

How to plan your first sequencing project
How to plan your first sequencing project

Designed for beginners, this NGS tutorial discusses experimental considerations and an overview of the Illumina sequencing workflow.

Learn about the Illumina NGS workflow
Learn about the Illumina NGS workflow

Illumina next-generation sequencing technology allows for massive parallel sequencing. Our experts will take you through Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.

Illumina sequencing: Intro to key customer resources
Illumina sequencing: Intro to key customer resources

This webinar discuss key resources available to customers for their NGS applications. This webinar is ideal for new users of Illumina NGS technologies, or experienced users that wish to learn more about the different resources Illumina offers.

Sequencing: An introduction

This webinar discusses the basics of DNA, fundamental concepts in DNA sequencing, and the applications of NGS as a research tool.

Sequencing fundamentals

This NGS tutorial covers the basics of DNA sequencing and how it compares to traditional methods.

Sequencing: Illumina technology

This course provides an overview of the Illumina sequencing workflow, from DNA extraction to the completion of a sequencing run.

Library prep tutorials for NGS

Preventing PCR contamination
Preventing PCR contamination

In this NGS tutorial, you’ll learn how to avoid a common source of contamination in biological experimentation.

How do I achieve consistent quantitation? Part 1
How do I achieve consistent quantitation? Part 1

Variation in DNA quantitation is a common dilemma in next-generation sequencing. Learn how these inconsistencies can arise.

How do I achieve consistent quantitation? Part 2
How do I achieve consistent quantitation? Part 2

This next-generation sequencing tutorial provides tips and tricks for getting reliable, consistent quantitation.

RNA sequencing part I: Introduction to Illumina RNA library preparation workflows
RNA sequencing part I: Introduction to Illumina RNA library preparation workflows

This video is an introduction to the Illumina RNA library prep workflows. Includes key considerations, kit selection, and sample preparation kit options for RNA input.

RNA sequencing part II: Best practices for Illumina RNA prep protocols
RNA sequencing part II: Best practices for Illumina RNA prep protocols

This video is an introduction to the Illumina RNA library prep workflows. Includes key considerations, kit selection, and sample preparation kit options for RNA input.

NGS bioinformatics tutorials

Introduction to sequencing data analysis
Introduction to sequencing data analysis

This NGS bioinformatics tutorial covers data analysis approaches and considerations for experimental design.

Introducing Illumina Connected Analytics
Introducing Illumina Connected Analytics

Analyze, manage, aggregate, and explore data at scale with Illumina Connected Analytics, a secure genomics data platform to operationalize informatics and drive scientific insights.

BaseSpace Sequence Hub: Introduction and analysis overview
BaseSpace Sequence Hub: Introduction and analysis overview

In this video, we discuss how to navigate the BaseSpace dashboard, general functionality, and tips for successful application and workflow utilization.

BaseSpace Informatics Suite: Introduction to Cohort Analyzer and Correlation Engine
BaseSpace Informatics Suite: Introduction to Cohort Analyzer and Correlation Engine

BaseSpace Informatics Suite simplifies genomic data management, analysis and reporting using a secure, scalable and open platform.

Illumina Connected Analytics - Interactive analysis for a single-cell RNA workflow
Illumina Connected Analytics - Interactive analysis for a single-cell RNA workflow

Watch how an Illumina Connected Analytics user can perform a single-cell RNA analysis workflow and interpret the results.

Enrichment data analysis: Introduction
Enrichment data analysis: Introduction

Illumina offers several enrichment-based library prep kits for targeted resequencing. This webinar will deconstruct enrichment data analysis for beginners.

Illumina experts: Analyzing and interpreting DNA methylation data
Illumina experts: Analyzing and interpreting DNA methylation data

Topics include epigenetic study design, methods for studying DNA methylation, bioinformatic analysis and interpretation of methylation data, and more.

NextSeq 1000/2000: Introduction to analysis with DRAGEN
NextSeq 1000/2000: Introduction to analysis with DRAGEN

This video discusses how to perform data analysis using DRAGEN on the NextSeq 1000/2000 platform.

RNA Sequencing: Part III - Introduction to analysis
RNA Sequencing: Part III - Introduction to analysis

In this video, we discuss the RNA-Seq analysis options available on BaseSpace including the RNA-Seq Alignment/Cufflinks, TopHat/Cufflinks, and RNA Express apps.

Additional resources

Resources and tools

A comprehensive roundup of useful links to help you at every step of your research— identifying the best approach, managing your workflow, purchasing what you need, and getting support.

Online training

Access on-demand training for instruments, library prep kits, software, and more.

How core labs can help you get started with NGS

In this on-demand webinar, NGS core facility scientists and researchers new to NGS discuss their fruitful collaborations, highlighting how to receive expert help from genomics facilities.

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