Advancements in Translational Biomarker Identification from Cell-Free RNA

November 3, 2021

Liquid biopsy is most often associated with cell-free DNA, but new research has shown additional value in interrogating a broader diversity of analytes in the blood. Cell-free RNA (cfRNA) is one such molecule that has been shown to reflect dynamic changes in many tissues. Researchers at Illumina have optimized key variables to enable scalable, end-to-end workflow solutions for whole-transcriptome library preparations of cfRNA. Continued application of cfRNA stands to benefit a multitude of human health concerns by empowering non-invasive interrogations of disease etiology and identification of predictive, diagnostic, and prognostic biomarkers, all of which will further facilitate our transition into the era of personalized medicine. #liquidbiopsy #rna #rnaseq #sequencing #transcription Resources: Paper referred to in video - RNA prep with Enrichment - Presenters: Dan Gheba – Executive Sequencing Specialist, Illumina Suzanne Rohrback PhD – Senior Scientist, Advanced Sciences, Illumina 00:00 Introduction to Liquid Biopsy 01:25 Why RNA over DNA? 04:24 Illumina cfRNA Study Introduction 05:43 cfRNA quality and method development 08:52 Sample Collection best practices 10:50 Study findings – unique biomarkers 14:24 Machine learning model 16:52 Method Update! 19:09 Publication Summary 20:28 How can you use cfRNA in your research? Subscribe to the Illumina video channel A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. *Data calculations on file. Illumina, Inc., 2015. View customer spotlight videos View Illumina webinars View Illumina product videos View Illumina support videos

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