DNA methylation analysis can help scientists gain valuable insight into gene regulation and identify potential biomarkers. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction. DNA methylation is also a common subject of agrigenomic investigations into responses to drought, temperature extremes, and other environmental changes.
High-throughput technologies such as next-generation sequencing (NGS) and microarrays enable genome-wide methylation profiling studies. These technologies offer new ways to understand the significance of DNA methylation, providing novel insights into the functional consequences of epigenetic variation.
Methylation Sequencing with NGS
NGS enables comprehensive profiling of methylation patterns at single-base resolution across the whole genome, or in targeted epigenetic regions of interest.
Arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.
|Most important to me||Comprehensive methylome coverage||High throughput (large sample numbers)|
|Least important to me||Throughput||Coverage|
~36 million CpGs (whole genome)
~3.3. million CpGs (targeted)
|3,000 – 850,000 CpGs|
All (whole genome)
Human & Mouse (flagship arrays)
All others (custom arrays)
|Learn More About Methylation Sequencing||Learn More About Methylation Arrays|
Genetic epidemiologist Dr. Kathleen C. Barnes discusses how methylation arrays add another dimension to the study of COVID-19 and other infectious diseases.Read Interview
Targeted methylation sequencing identifies epigenetic signatures that correlate with brain image scans of substance abusers.Read Interview
Researchers investigate the epigenetics behind cancer metastasis using DNA methylation microarrays and NGS.Read Interview
Studies of epigenetic alterations in cancer, such as aberrant methylation and transcription factor binding, can provide insight into important tumorigenic pathways. Learn more about cancer epigenetics research.
Combining methylation analysis and differential gene expression analysis can help scientists prioritize genes for further study and elucidate complex disease mechanisms. Learn more about gene target ID.
ATAC-Seq does not require prior knowledge of regulatory elements, making it a powerful epigenetic discovery tool. It is a popular method for determining chromatin accessibility across the genome. Learn more about ATAC-Seq.
Genome-wide association studies (GWAS) use high-throughput genomic techniques to quickly scan entire genomes of large groups to find genetic variants correlated with a trait or disease. Learn more about GWAS.