Now is the time to upgrade for more discovery power. Support and reagent availability for HiSeq 1000–3000 Systems ends February 28, 2023. Contact us to see if you qualify for a trade-in or use our Sequencing Platform Comparison Tool to find the best instrument for your needs.

TruSeq PE Cluster Kit v3-cBot-HS

This kit enables cluster generation for subsequent paired-end sequencing on select HiSeq Systems as well as the HiScanSQ System. Read More...
Select Product(s)

TruSeq PE Cluster Kit v3-cBot-HS



Product Highlights

For use with HiSeq 2500, HiSeq 2000, HiSeq 1000, and HiScanSQ instruments with the cBot System, the TruSeq PE (Paired-End) Cluster Kit v3-cBot-HS provides reagents that bind samples to complementary adapter oligos on paired-end flow cells. These enable copying of the DNA strand after the first sequencing run, and the copied strand is used to sequence the other fragment end.

This kit enables cluster amplification on the cBot System. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

Cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. The kit also includes reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.

Frequently Purchased Together

Best Practices for Storing Sequencing Reagents

Sequencing reagent performance depends on thawing technique, thawed storage time, and storage conditions. Learn how to thaw and store sequencing reagents for optimal performance.

View Bulletin
Best Practices for Storing Sequencing Reagents

Product Literature

Literature is not currently available for this product.

Illumina Performance Qualification

Verify instrument performance and obtain a digital, audit-ready PQ document from certified Illumina engineers to help meet regulatory requirements.