The HiSeq PE (Paired-End) Cluster Kit v4 cBot provides reagents for the cBot cluster amplification system. DNA library samples are bound to complimentary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridization primer by 3’ extension using a high fidelity DNA polymerase. Copies are isothermally amplified to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems*.
Cluster generation reagents provided in a pre-mixed, 96 well plate format that requires minimal reagent preparation. The kit also included reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.
If you currently own a HiSeq 2500, HiSeq 3000/4000, or HiSeq X, you may be eligible to trade in your existing instrument for a new NovaSeq 6000 System. This program is available for a limited time only. Terms and conditions apply. Fill out the form below to contact a sales representative to see if you are eligible.
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HiSeq Cluster Kit v4 Documentation
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Reagents for determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on enabled HiSeq systems.
The HiSeq SR (Single Read) Cluster Kit v4 cBot uses isothermal amplification to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems.
HiSeq Rapid Cluster Kits v2 enable an on-board cluster generation process, designed to be used in HiSeq Rapid Run mode.
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
HiSeq Rapid SBS Kits v2 are designed for the rapid run mode of HiSeq 2500 and 1500 sequencers.
