Sequencing Library Preparation Methods

Sequencing Method Explorer

Use this interactive tool to explore experimental NGS library prep methods compiled from the scientific literature. The tool includes a subset of methods from the review articles and posters below. New methods will be added as they become available.

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Sequencing Method Posters

View illustrated schematics of DNA, RNA, and single-cell library preparation techniques that are compatible with SBS technology. Also see brief diagrams of Illumina library preparation kit protocols.

For All You Seq—DNA

This poster includes NGS methods for analyzing:

  • DNA variants
  • Low levels of DNA
  • Epigenetics
  • DNA–protein interactions
  • Protein–protein interactions
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For All You Seq—RNA

This poster includes a comprehensive set of NGS methods for analyzing:

  • RNA transcription
  • RNA–protein interactions
  • Low levels of RNA
  • RNA modifications
  • RNA structure
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For All You Seq—Single-Cell

This poster includes a comprehensive set of NGS-based single-cell analysis methods for:

  • Low-level RNA detection
  • Low-level DNA detection
  • Integrated DNA and RNA analysis
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DNA and RNA Methods Reviews

Compare a Broad Range of Methods

Explore the world's largest collection of NGS library preparation techniques. These methods, compiled from the scientific literature, demonstrate the wide range of scientific questions you can address with Illumina sequencing by synthesis (SBS) technology.

These comprehensive review articles describe the various NGS methods, with pros and cons, publication summaries, and references.

Read DNA Methods ReviewRead RNA Methods Review

Featured Method


scTrio-Seq can analyze genomic CNVs, the DNA methylome, and the transcriptome of an individual mammalian cell simultaneousl. This approach is an extension of previous methods, such as scMT-seq.

  • Accurately analyzes the mechanism by which the transcriptome, genome, and DNA methylome regulate each other
  • CNVs can be identified reliably using scRRBS data
  • Lower transcriptome coverage than scMT-seq1
  • Results in 3'-biased transcriptome

SBS Technology

Learn more about the advantages of sequencing by synthesis, how it works, and the many ways you can leverage sequencing technology to help answer your research questions.

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Additional Resources


Photoactivatable ribonucleoside–enhanced crosslinking and immunoprecipitation (PAR-CLIP) maps RNA-binding proteins (RBPs). This approach is similar to HITS-CLIP and CLIP-Seq, but uses much more efficient crosslinking to stabilize the protein-RNA complexes. The requirement to introduce a photoactivatable ribonucleoside limits this approach to cell culture and in vitro systems. In this method, 4-thiouridine (4-SU) and 6-thioguanosine (6-SG) are incorporated into transcripts of cultured cells. UV irradiation crosslinks 4-SU/6-SG–labeled transcripts to interacting RBPs. The targeted complexes are immunoprecipitated and digested with RNase T1, followed by Proteinase K, before RNA extraction. The RNA is reverse-transcribed to cDNA and sequenced. Deep sequencing of cDNA accurately maps RBPs interacting with labeled transcripts.

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UMI Method