Library Prep Automation

Automation Methods that Span Our Library Prep Portfolio

Minimize errors, reduce hands-on time, and enable higher throughput

Illumina sequencing lab

Automate Your Library Prep

Automating library preparation can help increase your lab's efficiency and generate more consistent results. Automation can also help you maintain turnaround times, despite expanding sample workloads. To aid with adopting NGS automation, methods for many of our library prep assays are available from the leading automation companies. Our partners offer a range of automation platforms to address your laboratory's throughput needs.

Both Illumina and our partners have created methods to address the increasing range of automation products and library prep. Methods created by a partner are tested, distributed, and typically supported by that partner. Illumina-developed methods are distributed by Illumina and are usually supported by our partners.

Illumina Qualified Methods are available for several of our library prep kits. We verify that libraries created with these methods perform comparably to manually prepared libraries. These methods are developed by partners who test for functional performance and provide distribution and support. Under the Illumina Qualified program, our partners develop, test and support methods while Illumina provides technical guidance and data review only.

TruSight Qualified is a new category of automation methods where Illumina is more involved with the development, testing and support of oncology-based automated solutions. The goal is to provide an improved experience with automation and our oncology-based library prep products. TruSight Qualified methods are developed by a team consisting of both Illumina and partner automation specialists and are based on the design verification acceptance criteria of each assay. Increased testing and reproducibility studies help demonstrate and characterize the automated method performance. Some examples of additional testing include thermal profile studies for plate incubations, reagent stability and instrument-to-instrument reproducibility studies. To up-level the customer experience, Illumina is now providing first-line support for the TruSight Qualified methods. This includes determining the proper support channel, managing technical issue communications, and ensuring final resolution between Illumina, customers, and partners.

We make it easy to automate your library prep. The vendors below have methods designed for Illumina kits, eliminating the time and effort needed to develop your own method.

  Agilent Beckman Coulter Eppendorf Hamilton PerkinElmer Tecan
Illumina Stranded Total RNA   Biomek i7 epMotion 5075t NGS STAR Sciclone Fluent
Illumina Stranded mRNA     epMotion 5075t NGS STAR Sciclone  
Illumina RNA Prep with Enrichment   Biomek i7 epMotion 5075t NGS STAR Sciclone  
Illumina DNA PCR Free   Biomek i5
Biomek i7
epMotion 5075t NGS STAR Sciclone
Janus
Fluent
Illumina DNA Prep Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone Freedom EVO NGS
Illumina DNA Prep with Enrichment Bravo Option B Biomek iSeries epMotion 5075t NGS STAR Sciclone Freedom EVO NGS
Nextera XT DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone Freedom EVO NGS
AmpliSeq for Illumina*   Biomek i5 epMotion 5075t
epMotion 5073
NGS STAR
NGS STARlet
Sciclone  
TruSight Oncology 500HT   Biomek i7   NGS STAR with 96 MPH/SBS STAR MOA    
TruSight Oncology 500   Biomek NXp
Biomek i5
  NGS STAR/SBS STAR LSA    
TruSight Tumor 15   Biomek 4000 epMotion 5075t   Zephyr  
TruSight Tumor 170   Biomek NXp   NGS STAR    
TruSeq DNA PCR-Free Bravo Option B Biomek FXp epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq Nano DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq RNA Exome Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq RNA Fusion     epMotion 5075t NGS STAR    
TruSeq RNA Pan Cancer            
TruSeq RNA v2   Biomek FXp     Sciclone NGS and NGSx  
TruSeq Stranded mRNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq Stranded Total RNA Bravo Option B Biomek FXp epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
16S rRNA     epMotion 5075t
epMotion 5073
NGS STAR    

Illumina Qualified (Illumina analysis has shown that libraries prepared with these methods perform comparably to those prepared manually)

Documentation: user guide and / or app note available from our partners

TruSight Qualified (Co-develops, tests and triages initial support requests for these methods)

Starting in January 2022 Hamilton will refer to the NGS STAR configuration for TruSight Oncology library preparations as the SBS STAR LSA. All existing Illumina Qualified methods are backwards compatible and will continue to run on this system as this change is in name only.

*In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.

Frequently Purchased Together

Automated Library Prep Workflow Examples

Assessing Nucleic Acid Quality with the Agilent Fragment Analyzer System

The Fragment Analyzer accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing. System options accommodate both low- and high-throughput labs.

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Automated Nextera DNA Flex Library Prep (now Illumina DNA Prep) Workflow for High-Throughput Metagenomics

The streamlined, automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.

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Illumina high-throughput sequencing

Adopt High-Throughput Sequencing In Your Lab

High-throughput sequencing can cost-effectively run data-rich applications and process more samples to improve statistical signficance. Get rapid access to more data for greater insights.

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Library Prep Technical Tips

DNA/RNA Isolation Considerations

Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.

dsDNA Library Concentration

Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.

Quantification and Quality Control

Find out how to quantify and validate final libraries for a successful sequencing run.