Illumina Stranded mRNA Prep

A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA. Read More...
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Library Prep

Illumina® Stranded mRNA Prep, Ligation (16 Samples)



Illumina® Stranded mRNA Prep, Ligation (96 Samples)



Index Adapters

IDT® for Illumina® RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples)



IDT® for Illumina® RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples)



IDT® for Illumina® RNA UD Indexes Set C, Ligation (96 Indexes, 96 Samples)



IDT® for Illumina® RNA UD Indexes Set D, Ligation (96 Indexes, 96 Samples)




Illumina Stranded mRNA Library Prep Training


Product Highlights

Illumina Stranded mRNA Prep offers a streamlined RNA-Seq solution for clear and comprehensive analysis across the transcriptome. It offers extraordinary flexibility for input type and supports low input amounts, down to 25 ng total RNA. Illumina Stranded mRNA Prep enables precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as novel isoforms, gene fusions, and allele-specific expression.

Exceptional Performance

Illumina Stranded mRNA Prep is optimized to provide exceptional polyA capture efficiency and coverage uniformity. It minimizes the required sequencing depth for accurate, unbiased detection of the coding transcriptome. Illumina Stranded mRNA Prep is based on TruSeq ligation technology, which has been cited in over 9,926 publications since 2011.

Learn more about the technology

Flexibility for Many Study Designs

Illumina Stranded total RNA Prep supports a broad range of RNA inputs, from 25 ng to 1000 ng.*

Precise Measurement of Strand Orientation

This information enables detection of antisense transcription, enhances transcript annotation, and increases alignment efficiency.

Fast, Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Sequencing Scalability

Multiplex up to 384 samples and sequence in a single run. (Index Sets A and B are available now; Sets C and D are coming soon.)

The Power of RNA-Seq

RNA-Seq is increasingly the platform of choice for thought leaders, providing a detailed snapshot of the transcriptome at a given point in time. It offers numerous advantages over quantitative PCR, including:

  • Hypothesis-free experimental design, requiring no previous knowledge of the transcriptome 
  • Higher discovery power to detect known and novel transcripts 
  • Higher throughput capability to quantify hundreds to thousands of regions in each assay 
  • Broader dynamic range, providing more accurate measurement of gene expression 
  • More data per assay, providing full sequence and variant information

*Not compatible with FFPE samples

Frequently Purchased Together


Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System Mid Output: 5
High Output: 16
(based on 25M reads per sample)
2 × 75 bp
NextSeq 2000 System P2: 16
P3: 40
(based on 25M reads per sample)
2 × 75 bp
NovaSeq 6000 System SP: 32
S1: 64
S2: 164
S4: 384
(based on 25M reads per sample)
2 × 75 bp

Method-Specific Workflow Example


Supporting Data and Figures


Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

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