Illumina DNA Prep Training
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Illumina DNA Prep offers flexibility for many whole-genome sequencing applications.
Learn more about the technology
Illumina DNA Prep uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.
The Illumina DNA Prep workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*.
Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.
While accommodating various study requirements, the Illumina DNA Prep workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.
The IDT for Illumina - DNA/RNA UD Indexes Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now. Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.
Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.
For whole-genome sequencing, Illumina DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.
*Demonstrated protocols available.
**Customer-reported application
Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.
View Infographic
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | 1 sample per run (high output; based on 30× coverage of a human genome) | Up to 2 × 150 bp |
| NovaSeq 6000 System | 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome) | Up to 2 × 125 bp (rapid run) Up to 2 × 150 bp (high output) |
| Illumina DNA Prep | TruSeq DNA PCR-Free | TruSeq DNA Nano |
|---|
(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of Illumina sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.
Application Note | PDF 3 MB
An automated Nextera DNA Flex library preparation workflow for high-throughput metagenomics
Application Note | PDF < 1 MB
Nextera Crude Lysate Protocol for Metagenomic Whole-Genome Sequencing Studies
Application Note | PDF < 1 MB
Direct Bacterial Colony Sequencing with the Nextera™ DNA Flex Library Preparation Kit
Application Note | PDF 1 MB
Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry
Application Note | HTML
Nextera DNA Flex Library Preparation for Soil Shotgun Metagenomics Analysis
Application Note | HTML
Illumina library preparation solutions
Brochure | PDF 2 MB
Data Sheet | PDF < 1 MB
Illumina DNA Prep Support Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
This kit enables cluster generation for subsequent sequencing on the HiSeq 3000 and HiSeq 4000 Systems.
Kits include SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
Focused sequencing panel to assess 15 genes that are commonly mutated in solid tumors in a single assay, with a simple, rapid workflow.
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.
Assay targeting multiple variant types, including microsatellite instability (MSI) and tumor mutational burden (TMB).
