DNA methylation plays an important role in regulating gene expression. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction. DNA methylation is also a common subject of agrigenomic investigations into responses to drought, temperature extremes, and other environmental changes.
High-throughput technologies such as next-generation sequencing (NGS) and microarrays enable researchers to perform genome-wide methylation profiling. These technologies offer new ways to understand the significance of DNA methylation, providing novel insights into the functional consequences of variation.
Methylation Sequencing with NGS
NGS enables comprehensive profiling of methylation patterns at single-base resolution across the entire genome, for the highest clarity in epigenetic studies.
Arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.
|Most important to me||Complete methylome coverage||High throughput/large sample number|
|Least important to me||Throughput||Coverage|
|#CpGs covered||> 36 million||850,000|
|Learn More||Learn More|
Studies of epigenetic alterations in cancer, such as aberrant methylation and transcription factor binding, can provide insight into important tumorigenic pathways. As altered methylation often activates or silences genes, changes in the epigenome can affect gene expression and the rate of cancer progression. Learn more about cancer epigenetics.
Genome-wide methylation profiling can help researchers understand the functional mechanisms at work in complex disease cases. Aberrant cytosine methylation can impact gene expression, and has been implicated in many disease processes, such as Alzheimer’s disease and asthma. Learn more about complex disease research.