MiSeq Reagent Kit v2 | MiSeq Reagent Kit v3 | |||||
---|---|---|---|---|---|---|
Read Length | 1 × 36 bp | 2 × 25 bp | 2 × 150 bp | 2 × 250 bp | 2 × 75 bp | 2 × 300 bp |
Total Time* | ~4 hrs | ~5.5 hrs | ~24 hrs | ~39 hrs | ~21 hrs | ~56 hrs |
Output | 540–610 Mb | 750–850 Mb | 4.5–5.1 Gb | 7.5–8.5 Gb | 3.3–3.8 Gb | 13.2–15 Gb |
MiSeq Reagent Kit v2 Micro | MiSeq Reagent Kit v2 Nano | ||
---|---|---|---|
Read Length | 2 × 150 bp | 2 × 250 bp | 2 × 150 bp |
Total Time* | ~19 hrs | ~28 hrs | ~17 hrs |
Output | 1.2 Gb | 500 Mb | 300 Mb |
* Total time includes cluster generation, sequencing, and base calling on a MiSeq System enabled with dual-surface scanning.
MiSeq Reagent Kit v2 | MiSeq Reagent Kit v3 | MiSeq Reagent Kit v2 Micro | MiSeq Reagent Kit v2 Nano | |
---|---|---|---|---|
Single Reads | 12-15 million | 22–25 million | 4 million | 1 million |
Paired-End Reads | 24–30 million | 44–50 million | 8 million | 2 million |
** Install specifications based on Illumina PhiX control library at supported cluster densities (865-965 k/mm2 clusters passing filter for v2 chemistry and 1200-1400 k/mm2 clusters passing filter for v3 chemistry). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.
MiSeq Reagent Kit v2 | MiSeq Reagent Kit v3 |
---|---|
> 90% bases higher than Q30 at 1 × 36 bp | > 85% bases higher than Q30 at 2 × 75 bp |
> 90% bases higher than Q30 at 2 × 25 bp | > 70% bases higher than Q30 at 2 × 300 bp |
> 80% bases higher than Q30 at 2 × 150 bp | |
> 75% bases higher than Q30 at 2 × 250 bp |
† A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
The MiSeq System offers a DNA-to-results sequencing solution with a small footprint that fits into virtually any lab environment.
View Specification SheetThe MiSeq System harnesses proven Illumina SBS technology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
Paired-end sequencing enables both ends of the DNA fragment to be sequenced. Because the distance between each paired read is known, alignment algorithms can use this information to map the reads. This is especially helpful across difficult-to-sequence, repetitive regions of the genome.