The AmpliSeq for Illumina sequencing solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds of genes in a single run. AmpliSeq for Illumina panels are optimized for clinical cancer research and genetic disease studies.

TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons.Read More...
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TruSeq定制扩增子试剂盒(96 个样本)

FC-130-1001

配件产品
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TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)

FC-130-1003

TruSeq Custom Amplicon Filter Plate (1 plate)

FC-130-1006

TruSeq Index Plate Fixture & Collar Kit (2 each)

FC-130-1007

Infinium FFPE QC Kit (384 reactions)

WG-321-1001

Product Highlights

TruSeq定制扩增子试剂盒是一个完全可定制且基于扩增子的实验分析方法,适用于靶向重测序。该实验分析方法可让您专注于检测目标基因组的重点区域。其采用简单的工作流程,在单次反应中即可测序多达1536个扩增子。这种高度靶向的方法为发现、验证和筛查重点研究区域中的基因变异带来了前所未有的效率。

此试剂盒可用于多种样本类型,且仅需50纳克的输入基因组DNA(gDNA)。凭借每次反应对多达1536个扩增子进行多重分析的能力,其可覆盖高达650 kb的累积序列。此外,整合的双标签计划在每次运行中可支持多达96个样本,使您能够充分享受台式测序仪输出能力的益处。

该实验分析方法提供经改良的困难处理能力以应对高GC含量区域,此外文库产量、均匀性和稳定性也有所提高。此外,经优化的扩增步骤可使样本输入量要求从150纳克下降至50纳克。

简化的探针设计

使用免费易用、可提供最佳覆盖度的在线工具DesignStudio设计靶向TruSeq定制扩增子寡核苷酸探针。

了解DesignStudio的更多信息
立即在DesignStudio中启动项目 (需要登录)

简单的数据分析

其使用TruSeq扩增子BaseSpace应用程序实现自动化数据分析,可让研究人员跨多个运行轻松查看项目数据,包括检测到的变异。

物种兼容性

TruSeq定制扩增子支持人类、小鼠、大鼠和牛样本。DesignStudio设计算法还可用于玉米、水稻、猪、狗、大豆、鸡和羊样本;但是,由于生成的实验分析方法未经检验,因此无法保证其性能。此外,Illumina Concierge服务可针对任何目标物种设计定制实验分析方法和/或提供实验分析方法优化支持。

咨询Illumina Concierge服务

 

Find a list of automation vendors with robotic systems compatible with this kit

Frequently Purchased Together

规格

Method-Specific工作流程示例

 

客户故事

通过精准诊断制定个体化癌症治疗

Robert Daber博士使用TruSeq Custom Amplicon执行可报告预后和治疗选择的基因组检测。

了解更多
通过基因组学恢复并管理哥伦比亚河盆地历史鱼群

全新GT-Seq方法采用HiSeq系统降低基因分型成本,并可对数千条鱼进行分析。

了解更多

数据和图表支持

 
Comparison of TruSeq Custom Amplicon Kits
Technology TruSeq Custom Amplicon v1.5 TruSeq Custom Amplicon Low Input
Amplicon plexity (single pool) Up to 10,000 (via Concierge) Up to 1536
Input requirement 50 ng as low as 10 ng
FFPE Compatibility Yes – 250 ng Yes – 10 ng (FFPE DNA input dependent on quality)
Amplicon size 150, 175, 250, 425 bp 150, 175, 250 bp
Instrument MiniSeq, MiSeq, NextSeq MiniSeq, MiSeq, NextSeq
Read Length Up to 2 x 300 bp Up to 2 x 150 bp
Time 10 hours 6.5 hours
Kit size (samples) 96+ 16, 96+

Two TruSeq Custom Amplicon options for targeted sequencing are available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons. TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays.

相关产品

AmpliSeq for Illumina Custom DNA Panel

Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.


TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons.


Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.