The AmpliSeq for Illumina sequencing solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds of genes in a single run. AmpliSeq for Illumina panels are optimized for clinical cancer research and genetic disease studies.
The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
|Technology||TruSeq Custom Amplicon v1.5||TruSeq Custom Amplicon Low Input|
|Amplicon plexity (single pool)||Up to 10,000 (via Concierge)||Up to 1536|
|Input requirement||50 ng||as low as 10 ng|
|FFPE Compatibility||Yes – 250 ng||Yes – 10 ng (FFPE DNA input dependent on quality)|
|Amplicon size||150, 175, 250, 425 bp||150, 175, 250 bp|
|Instrument||MiniSeq, MiSeq, NextSeq||MiniSeq, MiSeq, NextSeq|
|Read Length||Up to 2 x 300 bp||Up to 2 x 150 bp|
|Time||10 hours||6.5 hours|
|Kit size (samples)||96+||16, 96+|
Two TruSeq Custom Amplicon options for targeted sequencing are available: TruSeq Custom Amplicon Low Input for low-input DNA and FFPE, or TruSeq Custom Amplicon 1.5 for very high plexity pools or longer amplicons. TruSeq Custom Amplicon Low Input enables you to detect and discover high-quality information, even from limited and challenging samples. Use the table to compare assays.