MiSeq Reagent Kits v2

Reagent kits that provide increased cluster density and read length for the MiSeq System, improving sequencing quality scores compared to v1 kits.

MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to enable long read lengths or a 150-cycle format that enables counting applications.

Overview

MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits but offer improvements in data quality and allow various formats for low-output applications. 

This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

  • Faster cycle times to deliver rapid and cost-effective turnaround time of sequencing applications

  • Twice the number of reads through dual surface imaging to support a wide range of applications

  • Standard, micro, and nano configurations enable alignment to specific project requirements and scalable levels of output

  • Radio-frequency identification (RFID)–encoded consumables to ensure reagent traceability and compatibility

MiSeq Reagent Kit 

Single readsa 

Paired-end readsa

Kit size (cycles) 

Output (max.) 

2 × 250 Output 

2 × 150 Output 

2 × 25 Output 

1 × 36 

Output 
MiSeq Reagent Kit v2  12–15M  24–30M 

50, 300, 500

8.5 Gb

7.5–8.5 Gb 

4.5–5.1 Gb 

750–850 Mb 

540–610 Mb 

MinSeq Reagent Micro Kit v2 

4M 

 8M 

300 

1.2 Gb 

N/A 

1.2 Gb 

N/A 

N/A 

MiSeq Reagent Nano Kit v2 

1M 

2M 

300, 500 

500 Mb 

500 Mb 

300 Mb 

N/A 

N/A 

a. Install specifications based on Illumina PhiX control library at supported cluster densities between 467–583 k/mm2 clusters passing filter for v2 chemistry and 727–827 k/mm2 clusters passing filter for v3 chemistry. Actual performance parameters may vary based on library type, library quality, and clusters passing filter.

Specifications

Instruments MiSeq System, MiSeqDx in Research Mode, MiSeq FGx in Research Mode
Nucleic acid type DNA, RNA
Reagent type Paired-End Sequencing, Cluster Generation, Sequencing by Synthesis
Technology Sequencing

Applications

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Documentation

Product literature
Support documentation

Resources

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MiSeq sequencing reagents preparation: Reagent cartridge

View a demonstration of the steps to prepare the MiSeq Reagent Cartridge prior to sequencing.

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MiSeq sequencing reagents preparation: Flow cell

View a demonstration of the steps to handle and clean the MiSeq flow cell prior to sequencing.

MiSeq Reagent Kit v2 (50-cycles)

MS-102-2001

Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 50-cycle run on the MiSeq System.

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MiSeq Reagent Kit v2 (300-cycles)

MS-102-2002

Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the MiSeq System.

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MiSeq Reagent Kit v2 (500-cycles)

MS-102-2003

Includes one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 500-cycle run on the MiSeq System.

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20-pack MiSeq Reagent Kit v2 (50-cycles)

MS-102-2021

Includes 20 kits each with one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support (20) 50-cycle runs on the MiSeq System.

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20-pack MiSeq Reagent Kit v2 (300-cycles)

MS-102-2022

Includes 20 kits each with one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support (20) 300-cycle runs on the MiSeq System.

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20-pack MiSeq Reagent Kit v2 (500-cycles)

MS-102-2023

Includes 20 kits each with one flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support (20) 500-cycle runs on the MiSeq System.

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MiSeq Reagent Micro Kit v2 (300-cycles)

MS-103-1002

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.

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MiSeq Reagent Nano Kit v2 (300-cycles)

MS-103-1001

Includes one Nano flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 300-cycle run on the MiSeq System.

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MiSeq Reagent Nano Kit v2 (500-cycles)

MS-103-1003

Includes one Nano flow cell and one pre-filled cartridge containing clustering and sequencing reagents to support a 500-cycle run on the MiSeq System.

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TG MiSeq Reagent Nano Kit, v2 (300 cycles)

TG-142-1001

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Nano Flow Cell (up to 1M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Nano Flow Cell, and Wash Buffer.

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TG MiSeq Reagent Micro Kit v2 (300 cycles)

TG-142-1002

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.

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TG MiSeq Reagent Kit, v2 (300 cycles)

TG-142-1003

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Standard Flow Cell (up to 15M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.

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TG MiSeq Reagent Kit v2 (500 cycles)

TG-142-1013

For TCG customers only - MiSeq v2 Reagent kit, 500 cycles per kit.

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TG MiSeq® Reagent Kit v2 (300 cycles) - 20 Pack

TG-142-1022

For TCG customers only - 20 pack of MiSeq v2 Reagent kits, 300 cycles per kit.

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TG MiSeq Reagent Nano Kit, v2 (300 cycles)

TG-142-1001

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Nano Flow Cell (up to 1M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Nano Flow Cell, and Wash Buffer.

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TG MiSeq Reagent Micro Kit v2 (300 cycles)

TG-142-1002

Provides kitted reagents for up to 325 cycles of sequencing on the MiSeq System using the MiSeq Micro Flow Cell (up to 4M clusters PF). Includes: Paired End Reagent plate (300-cycles), MiSeq Micro Flow Cell, and Wash Buffer.

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FAQ

MiSeq Reagent Kit v2 includes pre-filled, ready-to-use reagent cartridges for performing cluster generation and sequencing on the MiSeq System.

There are up to 15M reads per run.

MiSeq Reagent Kit v3 contains two additional reagents, the Incorporation Mix (IMT) and Scan Mix (USM), and the PR2 volume has been increased to 500ml to support longer runs. This ensures double the output of a single run using the v3 kits (3.3–15 Gb) compared to v2 kits (0.75–8.5 Gb).

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Trade in your HiSeq, MiSeq, or NextSeq 500/550 system

If you currently own a HiSeq, NextSeq 500/550, or a MiSeq System, you may be eligible to trade in your existing instrument for a NextSeq 1000 or NextSeq 2000 System. Restrictions apply. Contact a sales representative using the form below to learn more about eligibility.

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