MiSeq resources to further your research

Featured publications, system literature, videos, and other educational resources

MiSeq Resources

Somatic mutations in cerebral cortical malformations.

N Engl J Med 371 733-43 2014

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The Gut Microbiota of Rural Papua New Guineans: Composition, Diversity Patterns, and Ecological Processes.

Cell Rep   2015

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Development of a Comprehensive Sequencing Assay for Inherited Cardiac Condition Genes.

J Cardiovasc Transl Res 9 3-11 2016

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Find the right library prep kit or microarray, calculate sequencing coverage, explore methods, design custom assays, and more.

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Product Selection and Experiment Planning Tools
MiSeq System Guide

Information about instrument components, software, required products, performing a sequencing run, and instrument maintenance.

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MiSeq Site Prep Guide

Find lab specifications and requirements for preparing your lab for a MiSeq System.

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Sequencing Online Training

Online Illumina sequencing courses are free, interactive, and available any time.

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Personalized Training Workflow Selection Tool

This tool will help you determine the best hands-on sequencing training for your needs based on your area of interest, application, library preparation kit, and platform.

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Sequencing Coverage

Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.

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Quality Scores

Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.

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MiSeq Documentation

Find system guides, related application and technical notes, and other supporting documentation for the MiSeq System.

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Sequencing Troubleshooting Tips

These short videos provide expert tips for common sequencing issues such as overclustering and inconsistent quantitation.

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Custom Protocol Selector

The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

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Agriculture Throughout the World is Benefiting from the Latest NGS Technologies

Texas A&M AgriLife uses the NovaSeq 6000 and iSeq 100 Systems to provide high-quality plant, animal and insect genome data to its partners worldwide.

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Scaling from Exome to Whole-Genome Sequencing with the DRAGEN Bio-IT Platform

The DRAGEN platform enables GeneDx to scale to whole-genome analysis and identify variants with precision.

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Using the MiSeq for Microbial Sequencing Applications
Using the MiSeq for Microbial Sequencing Applications

Practical information for new MiSeq users with many tips, tricks, and microbial examples gleaned from long instrument experience.

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Investigating Tree Sloths with Mixed Amplicon Sequencing
Investigating Tree Sloths with Mixed Amplicon Sequencing

Researchers used paired-end 16S amplicon sequencing on the MiSeq System to explore their microbe-cycling hypothesis.

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Ebola and Sexual Transmission
Ebola and Sexual Transmission

Researchers compared viral RNA sequences from samples, and found substitutions absent from other Western African Ebola genomes.

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