Discuss best practices, troubleshoot, and learn about how others are using Illumina sequencers, library preparation kits, and automated data analysis to fuel their research. Collaborate with Illumina customers, moderators, and developers.
The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports a broad range of applications and study sizes. Choose between rapid run and high output modes to fit your project needs.
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 250 base pair reads. The longer reads enable greater depth of coverage, improved assembly for de novo applications and aid in long-read applications such as metagenomic analyses.
High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process over 6× more samples than rapid mode and to complete large projects quickly and efficiently. HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.
Learn how DNA sequencing is leading to progress in the rapid identification of variants that might be involved in genetic diseases. Thanks to this work, Dr. Kingsmore and his team now hold a Guinness World Records title.View Video
Researchers in India study a deadly oral cancer that is strongly associated with chewing tobacco together with slaked lime.Read Article
Landmark NGS study leads to a national clinical diagnostic exome sequencing platform in Singapore.Read Article
HiSeq Rapid v2 SBS Kits enable the longest reads on HiSeq instruments, with reads up to 2x250 bp. The new kits generate up to 300 Gb of data per run. HiSeq Rapid SBS Kits v2 are the ideal choice for sequencing on HiSeq systems.Learn More
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
Reagents for determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on enabled HiSeq systems.