At the start of a project, a project manager contacts the PI and walks them through the sequencing service process. Each sample is handled with care from arrival at Illumina through data generation. Samples meeting the DNA requirements are processed through FastTrack Services sequencing workflows using Illumina HiSeq technology.
Throughout the process, there are multiple QC steps to give our customers confidence in their data. Illumina FastTrack Services offers a number of service packages, including whole-genome sequencing (WGS) services and cancer analysis services.
The value of Illumina FastTrack Services Process:
Illumina FastTrack Services has a high-throughput, automated process to achieve best delivery of every whole genome sequenced. The chart below depicts how your project will be handled, from sample receipt through data delivery. You will notice that we have several QC steps embedded in our process. Each step allows us to verify that your samples will run through our pipeline smoothly, and ensure that you will be provided with the highest quality data available.
Illumina FastTrack Services offers human WGS services with a turnaround time of 8 weeks for 90% of project sizes <200 samples for standard 30X WGS. For some projects, approximately 10% of the samples may require further investigation. Our project managers will work with each customer to investigate samples and determine next steps. The ultimate goal is to ensure the success of every project.
FastTrack Services also offers a comprehensive cancer analysis service to interrogate tumor-normal datasets for somatic variants.
|Standard Human WGS||Cancer Analysis Add-On|
|Sample Requirement||2 ug||Included in WGS requirement|
|Library Preparation Method||TruSeq DNA PCR-free||Included in WGS requirement|
|Coverage||30× coverage or ≥ 100Gb data to 100× coverage or ≥ 330Gb data||40× coverage or ≥ 135Gb data for normal sample
80× coverage or ≥ 270Gb data for tumor sample
|Sequencing Parameters||Paired-end, minimum 2×100bp reads||Paired-end, minimum 2×100bp reads|
|Turnaround Time||For project sizes of 200 samples or less, 90% of a project is completed within 8 weeks. Contact an Illumina sales representative for an up-to-date turnaround time.||For project sizes of 200 samples or less, 90% of a project is completed within 8 weeks. Contact an Illumina sales representative for an up-to-date turnaround time.|
|Deliverable||WGS analysis includes consensus sequence, called SNPs, Indels, SVs, CNVs and array data in archival BAM, VCF and gVCF formats. Dataset includes all reads and quality scores for downstream analysis.||Cancer analysis includes genomic comparison files for somatic SNPs, indels, SVs, & CNAs in VCF format. Dataset includes all reads and quality scores for downstream analysis.|