The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.
Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.
Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.
Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.
View sample data (requires a BaseSpace login)
This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
NextSeq 550 System | 4 to 6 samples per run (based on a minimum of 100,000 reads per cell) | read 1: 68 bp, read 2: 75 bp |
HiSeq 2500 System | Rapid run: 4-5 samples per lane; high output v4: 4 samples per lane (based on a minimum of 100,000 reads per cell) | read 1: 68 bp, read 2: 75 bp |
SureCell WTA 3′ Library Prep Kit for the ddSEQ System | TruSeq RNA Library Prep Kit v2 | Illumina Stranded mRNA Prep | Illumina RNA Prep with Enrichment | |
---|---|---|---|---|
Assay Time | 11-12 hours | ~10.5 hours | 6.5 hours | < 9 hours |
Content Specifications | Captures the coding transcriptome (3' enriched), with strand-specific information | Captures the coding transcriptome (without strand information) | Captures the coding transcriptome with strand information | Captures the coding transcriptome when used with Illumina Exome Panel |
Description | Enables single-cell RNA-Seq studies using a droplet generator that isolates ~1200 cells per cartridge, and a simple library prep process. | A simple, cost-effective research solution for analysis of the coding transcriptome. | A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information | A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples |
Hands-On Time | 4-5 hours | ~4.5 hours | < 3 hours | < 2 hours |
Input Quantity | ~45,000 cells per cartridge (required input) | 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) | 25-1000 ng standard-quality total RNA | 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples |
Mechanism of Action | Oligo dT capture, single cell | Oligo-dT beads capture polyA tails | PolyA capture, ligation-based addition of adapters and indexes | Bead-linked transposome |
Method | mRNA Sequencing | mRNA Sequencing | mRNA Sequencing | mRNA Sequencing, Target Enrichment, Targeted RNA Sequencing |
Multiplexing | 1-24 | Up to 24-plex per lane | Up to 384 Unique Dual Indexes (UDIs) | Up to 384 Unique Dual Indexes (UDIs) |
Specialized Sample Types | Not FFPE-Compatible, Single Cells | Not FFPE-Compatible | Low-Input Samples, Not FFPE-Compatible | Blood, FFPE Tissue, Low-Input Samples |
Species Category | Human, Mouse | Bovine, Human, Mammalian, Mouse, Rat | Bovine, Human, Mammalian, Mouse, Rat | Human, Virus |
Strand Specificity | Stranded | Non-Stranded | Stranded | Non-Stranded |
Illumina | Bio-Rad Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells
Application Note | PDF 1 MB
Single-Cell RNA Data Analysis Workflow
Technical Note | PDF < 1 MB
Illumina Bio-Rad Single-Cell RNA Sequencing Solution
Data Sheet | PDF < 1 MB
SureCell WTA 3' Library Prep Kit Documentation
Custom Protocol Selector
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