The AmpliSeq for Illumina Exome Panel brings the speed and simplicity of PCR to exome sequencing, enabling researchers to sequence eight exomes in a single run and identify germline variants in less time. Key features include:
The Exome Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
NextSeq 550 System | Samples per run: high output: 8 samples per run (based on >95% covered at 30×) | 2 x 151 bp |
AmpliSeq for Illumina Exome Panel | TruSeq DNA Exome | Illumina DNA Prep with Enrichment | |
---|---|---|---|
Assay Time | < 7.5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 2.5 days | ~6.5 hours |
Content Specifications | > 97% of CCDS | Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). | Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel. |
Description | Enables researchers to prepare 8 exome libraries in <7.5 hours with <1.5 hours hands-on time. | A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content. | A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing. |
Hands-On Time | < 1.5 hours | 6 hours | ~2 hours |
Input Quantity | 50 ng minimum | 100 ng genomic DNA | 10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide). |
Mechanism of Action | Multiplex PCR | Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes | Bead-bound transposomes and hybrid-capture chemistry |
Method | Amplicon Sequencing, Exome Sequencing | Exome Sequencing | Custom Sequencing, Exome Sequencing, Target Enrichment, Targeted DNA Sequencing |
Multiplexing | 96 dual index combinations | Up to 12-plex enrichment | Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested. |
On-Target Reads | >80% | >80% | ≥85% (for exome panel)* |
Specialized Sample Types | Not FFPE-Compatible | FFPE Tissue | Blood, FFPE Tissue, Saliva |
Species Category | Human | Human | Human, Other |
Uniformity | >90% (% coverage at 20x for 4 Gb) | >85% (% coverage at 20x for 4 Gb) | ≥90% (% coverage at 20x for 4 Gb; for exome panel) |
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
Sample Identification with the AmpliSeq for Illumina Sample ID Panel Application Note
Application Note | HTML
AmpliSeq for Illumina Exome Panel Data Sheet
Data Sheet | HTML
AmpliSeq for Illumina Exome Panel Gene List
product_file | EXCEL < 1 MB
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Exome Panel Documentation
AmpliSeq for Illumina Exome Panel Reference Guide Documentation
AmpliSeq for Illumina Exome Panel Consumables & Equipment Documentation
AmpliSeq for Illumina Exome Panel Checklist Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions