TruSight Cardio Sequencing Kit

This kit provides researchers with a comprehensive, cost-effective solution for identifying causal variants implicated in inherited cardiac conditions (ICCs).Read More...
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Bundled Solutions

TruSight Cardio Sequencing Kit for MiSeq and MiSeqDx (12 indexes, 12 samples, 1 enrichment)

FC-141-1010

Price
 
 

TruSight Cardio Sequencing Kit for NextSeq (48 indexes, 48 samples, 4 enrichments)

FC-141-1011

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Enrichment Panels

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229

Price
 
 
Accessory Products

Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 96 samples (8, 12-plex enrichment reactions)

20025524

Price
 
 

Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents - 16 samples (16, 1-plex enrichment reactions)

20025523

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (96 samples)

20025520

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Nextera DNA Flex Pre-Enrichment Library Prep Reagents (16 samples)

20025519

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set A (96 Indexes, 96 Samples)

20027213

Price
 
 

Product Highlights

  • Expertly defined genes selected in collaboration with the Imperial College of London
  • 99% of the targeted regions covered at a depth of at least 20×*
  • Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiSeq System 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)
NextSeq 550 System 48 samples per run (mid output; based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)

Product Comparison

TruSight Cardio Sequencing Kit TruSight One Sequencing Panels TruSight Inherited Disease
Content Specifications 575 Kb genomic content (174 genes) TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
2.25 Mb genomic content (552 genes)
Description Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions. Fixed research panels targeting exonic regions that harbor disease-causing variants. Fixed research panel focused on severe pediatric onset disorders.
Input Quantity 50 ng DNA 50 ng DNA 50 ng DNA
Method Target Enrichment , Targeted DNA Sequencing Target Enrichment , Targeted DNA Sequencing Target Enrichment , Targeted DNA Sequencing
Multiplexing Up to 96-plex Up to 96-plex Up to 96-plex
Specialized Sample Types Not FFPE-Compatible Not FFPE-Compatible Not FFPE-Compatible
Species Category Human Human Human

Method-Specific Workflow Example

 

The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.

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References
  1. Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Trans Res. 2016;9:3.

* Data on file. Illumina, Inc. 2015.