Details

  • A time-saving solution that is 85% faster than the standard Illumina library prep and enrichment
  • Integrated DNA extraction protocols for blood and saliva
  • Optimized performance using bead-based library prep chemistry for reliable results

Compatibility with custom, fixed, or whole exome panels from Illumina or 3rd party oligo vendors Illumina DNA Prep with Enrichment uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time. From DNA input and extraction to library normalization, enrichment, and post-enrichment amplification, the total assay time is ~ 6.5 hours, and total hands-on time is ~2 hours.

 Illumina DNA Prep with Enrichment supports a broad DNA input range (10-1000 ng high-quality genomic DNA or 50 ng FFPE DNA) and multiple sample types, including blood, saliva, genomic DNA, and formalin-fixed, paraffin-embedded (FFPE) tissue. The Flex Lysis Reagent Kit enables fast DNA extraction from blood and saliva samples to go directly into library prep, reducing the number of required quantification steps to maximize cost-effectiveness. While accommodating various study requirements, the Illumina DNA Prep with Enrichment solution delivers consistent insert sizes, uniform coverage, optimized performance, and highly reproducible data across all Illumina sequencing systems.

Location: Princess Margaret Hospital (Toronto)

Date & Time
May 2, 2019
Location
Princess Margaret Hospital, Toronto, Canada
North America
Topic
Library Preparation Kits
This event has passed