DNA analysis methods for human identification with NGS

Forensic DNA Analysis Methods

Today, most forensic DNA testing utilizes PCR and capillary electrophoresis (CE)-based analysis methods to detect fragment length variation in short tandem repeat (STR) markers. A small percentage of forensic investigations also use CE-based Sanger sequencing to analyze specific regions of mitochondrial DNA (mtDNA).

Now forensic scientists can harness the full power of targeted next-generation sequencing (NGS). Illumina sequencing by synthesis (SBS) technology offers a massively parallel approach for sequencing PCR amplicons. Our fully validated MiSeqFGx Forensic Genomics System offers the high accuracy and ease of use that will set a new gold standard for forensic DNA testing.

MiSeq FGx System
ForenSeq SNP and STR Sequencing on the MiSeq FGx

Targeted sequencing of forensically relevant loci relieves many of the limitations of genotyping based on fragment length detection. NGS enables single nucleotide polymorphism (SNP) and STR analysis for large numbers of globally relevant STR markers and dense SNP sets in a single forensic DNA test.

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Forensic mtDNA Sequencing

Because mtDNA is present in hundreds of copies per cell, it can survive environments where nuclear DNA does not, and can be a powerful tool for human identification. Illumina NGS provides deep coverage to extract genetic data from small forensic DNA samples found in less than ideal condition.

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